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Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy

BACKGROUND: Preliminary data suggest that the urinary microbiome may play a role in bladder cancer. Information regarding the most suitable method of collecting urine specimens is needed for the large population studies needed to address this. To compare microbiome metrics resulting from 16S ribosom...

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Autores principales: Hourigan, Suchitra K., Zhu, Wei, S.W.Wong, Wendy, Clemency, Nicole C., Provenzano, Marina, Vilboux, Thierry, Niederhuber, John E., Deeken, John, Chung, Simon, McDaniel-Wiley, Kim, Trump, Donald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986141/
https://www.ncbi.nlm.nih.gov/pubmed/31992287
http://dx.doi.org/10.1186/s12894-020-0576-z
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author Hourigan, Suchitra K.
Zhu, Wei
S.W.Wong, Wendy
Clemency, Nicole C.
Provenzano, Marina
Vilboux, Thierry
Niederhuber, John E.
Deeken, John
Chung, Simon
McDaniel-Wiley, Kim
Trump, Donald
author_facet Hourigan, Suchitra K.
Zhu, Wei
S.W.Wong, Wendy
Clemency, Nicole C.
Provenzano, Marina
Vilboux, Thierry
Niederhuber, John E.
Deeken, John
Chung, Simon
McDaniel-Wiley, Kim
Trump, Donald
author_sort Hourigan, Suchitra K.
collection PubMed
description BACKGROUND: Preliminary data suggest that the urinary microbiome may play a role in bladder cancer. Information regarding the most suitable method of collecting urine specimens is needed for the large population studies needed to address this. To compare microbiome metrics resulting from 16S ribosomal RNA gene sequencing between midstream, voided specimens and those obtained at cystoscopy. METHODS: Adults, with a history of superficial urothelial cell carcinoma (non-muscle invasive bladder cancer) being followed with periodic surveillance cystoscopy had a urine sample collected by a mid-stream, voided technique and then from the bladder at cystoscopy. Urine samples underwent 16S ribosomal RNA gene sequencing on the Illumina MiSeq platform. RESULTS: 22 subjects (8 female, 14 male) were included. There was no significant difference in beta diversity (diversity between samples) in all samples between collection methods. However, analysis by sex revealed a difference between voided and cystoscopy samples from the same individual in males (p = 0.006, Adonis test) but not in females (p = 0.317, Adonis test). No differences were seen by collection method in any alpha diversity (diversity within a sample) measurement or differential abundance of taxa. CONCLUSIONS: Beta diversity of the urine microbiome did differ by collection method for males only. This suggests that the urinary microbiomes of the two collection methods are not equivalent to each other, at least in males, which is the sex that bladder cancer occurs most frequently in. Therefore, the same collection method within a given study should be used.
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spelling pubmed-69861412020-01-30 Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy Hourigan, Suchitra K. Zhu, Wei S.W.Wong, Wendy Clemency, Nicole C. Provenzano, Marina Vilboux, Thierry Niederhuber, John E. Deeken, John Chung, Simon McDaniel-Wiley, Kim Trump, Donald BMC Urol Research Article BACKGROUND: Preliminary data suggest that the urinary microbiome may play a role in bladder cancer. Information regarding the most suitable method of collecting urine specimens is needed for the large population studies needed to address this. To compare microbiome metrics resulting from 16S ribosomal RNA gene sequencing between midstream, voided specimens and those obtained at cystoscopy. METHODS: Adults, with a history of superficial urothelial cell carcinoma (non-muscle invasive bladder cancer) being followed with periodic surveillance cystoscopy had a urine sample collected by a mid-stream, voided technique and then from the bladder at cystoscopy. Urine samples underwent 16S ribosomal RNA gene sequencing on the Illumina MiSeq platform. RESULTS: 22 subjects (8 female, 14 male) were included. There was no significant difference in beta diversity (diversity between samples) in all samples between collection methods. However, analysis by sex revealed a difference between voided and cystoscopy samples from the same individual in males (p = 0.006, Adonis test) but not in females (p = 0.317, Adonis test). No differences were seen by collection method in any alpha diversity (diversity within a sample) measurement or differential abundance of taxa. CONCLUSIONS: Beta diversity of the urine microbiome did differ by collection method for males only. This suggests that the urinary microbiomes of the two collection methods are not equivalent to each other, at least in males, which is the sex that bladder cancer occurs most frequently in. Therefore, the same collection method within a given study should be used. BioMed Central 2020-01-28 /pmc/articles/PMC6986141/ /pubmed/31992287 http://dx.doi.org/10.1186/s12894-020-0576-z Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hourigan, Suchitra K.
Zhu, Wei
S.W.Wong, Wendy
Clemency, Nicole C.
Provenzano, Marina
Vilboux, Thierry
Niederhuber, John E.
Deeken, John
Chung, Simon
McDaniel-Wiley, Kim
Trump, Donald
Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title_full Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title_fullStr Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title_full_unstemmed Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title_short Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
title_sort studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986141/
https://www.ncbi.nlm.nih.gov/pubmed/31992287
http://dx.doi.org/10.1186/s12894-020-0576-z
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