Cargando…
MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36
BACKGROUND/AIMS: The effects of microRNA-423 on proliferation and drug resistance of breast cancer cells were explored, the downstream target genes of miR-423 and the targeted regulatory relationship between them were studied. METHODS: RT-qPCR was used to detect the expression of miR-423 in breast c...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986407/ https://www.ncbi.nlm.nih.gov/pubmed/32158228 http://dx.doi.org/10.2147/OTT.S217745 |
_version_ | 1783491959867834368 |
---|---|
author | Xia, Wenfei Liu, Yun Du, Yaying Cheng, Teng Hu, Xiaopeng Li, Xingrui |
author_facet | Xia, Wenfei Liu, Yun Du, Yaying Cheng, Teng Hu, Xiaopeng Li, Xingrui |
author_sort | Xia, Wenfei |
collection | PubMed |
description | BACKGROUND/AIMS: The effects of microRNA-423 on proliferation and drug resistance of breast cancer cells were explored, the downstream target genes of miR-423 and the targeted regulatory relationship between them were studied. METHODS: RT-qPCR was used to detect the expression of miR-423 in breast cancer tissues and cell lines, and the transfection efficiency of miR-423 inhibitory vector miR-423-inhibitor was constructed and verified. CCK-8 and colony formation assays were used to examine the effect of miR-423 on tumor cell proliferation. Target gene prediction and screening and luciferase reporter assay were used to verify downstream target genes of miR-432. The mRNA and protein expression of miR-423target gene ZFP36 was detected by RT-qPCR and Western blotting. RESULTS: The expression of miR-423 was significantly higher than that in normal tissues. Compared to the non-malignant mammary epithelial cell line MCF-10A, the expression of miR-423 was significantly raised in MCR-7 and MCF-7/ADR cells. ZFP36 was a downstream target gene of miR-423 and negatively correlated with the expression of miR-423 in breast cancer. The knockdown of miR-423 can significantly enhance the cytotoxicity of the drug, increase the apoptotic rate of MCF-7/ADR cells. miR-423 was capable of activating the Wnt/β-catenin signaling pathway leading to chemoresistance and proliferation, whereas overexpression of ZFP36 reduced drug resistance and proliferation. CONCLUSION: miR-423 acted as an oncogene to promote tumor cell proliferation and migration. ZFP36 was a downstream target gene of miR-423, and miR-423 inhibited the expression of ZFP36 via Wnt/β-catenin signaling pathway of breast cancer cells. |
format | Online Article Text |
id | pubmed-6986407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69864072020-03-10 MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 Xia, Wenfei Liu, Yun Du, Yaying Cheng, Teng Hu, Xiaopeng Li, Xingrui Onco Targets Ther Original Research BACKGROUND/AIMS: The effects of microRNA-423 on proliferation and drug resistance of breast cancer cells were explored, the downstream target genes of miR-423 and the targeted regulatory relationship between them were studied. METHODS: RT-qPCR was used to detect the expression of miR-423 in breast cancer tissues and cell lines, and the transfection efficiency of miR-423 inhibitory vector miR-423-inhibitor was constructed and verified. CCK-8 and colony formation assays were used to examine the effect of miR-423 on tumor cell proliferation. Target gene prediction and screening and luciferase reporter assay were used to verify downstream target genes of miR-432. The mRNA and protein expression of miR-423target gene ZFP36 was detected by RT-qPCR and Western blotting. RESULTS: The expression of miR-423 was significantly higher than that in normal tissues. Compared to the non-malignant mammary epithelial cell line MCF-10A, the expression of miR-423 was significantly raised in MCR-7 and MCF-7/ADR cells. ZFP36 was a downstream target gene of miR-423 and negatively correlated with the expression of miR-423 in breast cancer. The knockdown of miR-423 can significantly enhance the cytotoxicity of the drug, increase the apoptotic rate of MCF-7/ADR cells. miR-423 was capable of activating the Wnt/β-catenin signaling pathway leading to chemoresistance and proliferation, whereas overexpression of ZFP36 reduced drug resistance and proliferation. CONCLUSION: miR-423 acted as an oncogene to promote tumor cell proliferation and migration. ZFP36 was a downstream target gene of miR-423, and miR-423 inhibited the expression of ZFP36 via Wnt/β-catenin signaling pathway of breast cancer cells. Dove 2020-01-24 /pmc/articles/PMC6986407/ /pubmed/32158228 http://dx.doi.org/10.2147/OTT.S217745 Text en © 2020 Xia et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Xia, Wenfei Liu, Yun Du, Yaying Cheng, Teng Hu, Xiaopeng Li, Xingrui MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title | MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title_full | MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title_fullStr | MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title_full_unstemmed | MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title_short | MicroRNA-423 Drug Resistance and Proliferation of Breast Cancer Cells by Targeting ZFP36 |
title_sort | microrna-423 drug resistance and proliferation of breast cancer cells by targeting zfp36 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986407/ https://www.ncbi.nlm.nih.gov/pubmed/32158228 http://dx.doi.org/10.2147/OTT.S217745 |
work_keys_str_mv | AT xiawenfei microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 AT liuyun microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 AT duyaying microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 AT chengteng microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 AT huxiaopeng microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 AT lixingrui microrna423drugresistanceandproliferationofbreastcancercellsbytargetingzfp36 |