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Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia

Escherichia coli (E. coli) from the B2 phylogenetic group is implicated in colorectal cancer (CRC) as it possesses a genomic island, termed polyketide synthetase (pks), which codes for the synthesis of colibactin, a genotoxin that induces DNA damage, cell cycle arrest, mutations and chromosomal inst...

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Autores principales: Iyadorai, Thevambiga, Mariappan, Vanitha, Vellasamy, Kumutha Malar, Wanyiri, Jane Wangui, Roslani, April Camilla, Lee, Goh Khean, Sears, Cynthia, Vadivelu, Jamuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986756/
https://www.ncbi.nlm.nih.gov/pubmed/31990962
http://dx.doi.org/10.1371/journal.pone.0228217
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author Iyadorai, Thevambiga
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Wanyiri, Jane Wangui
Roslani, April Camilla
Lee, Goh Khean
Sears, Cynthia
Vadivelu, Jamuna
author_facet Iyadorai, Thevambiga
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Wanyiri, Jane Wangui
Roslani, April Camilla
Lee, Goh Khean
Sears, Cynthia
Vadivelu, Jamuna
author_sort Iyadorai, Thevambiga
collection PubMed
description Escherichia coli (E. coli) from the B2 phylogenetic group is implicated in colorectal cancer (CRC) as it possesses a genomic island, termed polyketide synthetase (pks), which codes for the synthesis of colibactin, a genotoxin that induces DNA damage, cell cycle arrest, mutations and chromosomal instability in eukaryotic cells. The aim of this study was to detect and compare the prevalence of E. coli expressing pks (pks(+) E. coli) in CRC patients and healthy controls followed by investigating the virulence triggered by pks(+) E. coli using an in-vitro model. Mucosal colon tissues were collected and processed to determine the presence of pks(+) E. coli. Thereafter, primary colon epithelial (PCE) and colorectal carcinoma (HCT116) cell lines were used to detect cytopathic response to the isolated pks(+) E. coli strains. Our results showed 16.7% and 4.3% of CRC and healthy controls, respectively were pks(+) E. coli. Further, PCE displayed syncytia and cell swelling and HCT116 cells, megalocytosis, in response to treatment with the isolated pks(+) E. coli strains. In conclusion, pks(+) E. coli was more often isolated from tissue of CRC patients compared to healthy individuals, and our in-vitro assays suggest these isolated strains may be involved in the initiation and development of CRC.
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spelling pubmed-69867562020-02-18 Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia Iyadorai, Thevambiga Mariappan, Vanitha Vellasamy, Kumutha Malar Wanyiri, Jane Wangui Roslani, April Camilla Lee, Goh Khean Sears, Cynthia Vadivelu, Jamuna PLoS One Research Article Escherichia coli (E. coli) from the B2 phylogenetic group is implicated in colorectal cancer (CRC) as it possesses a genomic island, termed polyketide synthetase (pks), which codes for the synthesis of colibactin, a genotoxin that induces DNA damage, cell cycle arrest, mutations and chromosomal instability in eukaryotic cells. The aim of this study was to detect and compare the prevalence of E. coli expressing pks (pks(+) E. coli) in CRC patients and healthy controls followed by investigating the virulence triggered by pks(+) E. coli using an in-vitro model. Mucosal colon tissues were collected and processed to determine the presence of pks(+) E. coli. Thereafter, primary colon epithelial (PCE) and colorectal carcinoma (HCT116) cell lines were used to detect cytopathic response to the isolated pks(+) E. coli strains. Our results showed 16.7% and 4.3% of CRC and healthy controls, respectively were pks(+) E. coli. Further, PCE displayed syncytia and cell swelling and HCT116 cells, megalocytosis, in response to treatment with the isolated pks(+) E. coli strains. In conclusion, pks(+) E. coli was more often isolated from tissue of CRC patients compared to healthy individuals, and our in-vitro assays suggest these isolated strains may be involved in the initiation and development of CRC. Public Library of Science 2020-01-28 /pmc/articles/PMC6986756/ /pubmed/31990962 http://dx.doi.org/10.1371/journal.pone.0228217 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Iyadorai, Thevambiga
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Wanyiri, Jane Wangui
Roslani, April Camilla
Lee, Goh Khean
Sears, Cynthia
Vadivelu, Jamuna
Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title_full Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title_fullStr Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title_full_unstemmed Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title_short Prevalence and association of pks(+) Escherichia coli with colorectal cancer in patients at the University Malaya Medical Centre, Malaysia
title_sort prevalence and association of pks(+) escherichia coli with colorectal cancer in patients at the university malaya medical centre, malaysia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986756/
https://www.ncbi.nlm.nih.gov/pubmed/31990962
http://dx.doi.org/10.1371/journal.pone.0228217
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