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Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness

Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced...

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Autores principales: McLaughlin, Heather P., Bugrysheva, Julia V., Conley, Andrew B., Gulvik, Christopher A., Cherney, Blake, Kolton, Cari B., Marston, Chung K., Saile, Elke, Swaney, Erin, Lonsway, David, Gargis, Amy S., Kongphet-Tran, Thiphasone, Lascols, Christine, Michel, Pierre, Villanueva, Julie, Hoffmaster, Alex R., Gee, Jay E., Sue, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986837/
https://www.ncbi.nlm.nih.gov/pubmed/31961318
http://dx.doi.org/10.3201/eid2602.191351
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author McLaughlin, Heather P.
Bugrysheva, Julia V.
Conley, Andrew B.
Gulvik, Christopher A.
Cherney, Blake
Kolton, Cari B.
Marston, Chung K.
Saile, Elke
Swaney, Erin
Lonsway, David
Gargis, Amy S.
Kongphet-Tran, Thiphasone
Lascols, Christine
Michel, Pierre
Villanueva, Julie
Hoffmaster, Alex R.
Gee, Jay E.
Sue, David
author_facet McLaughlin, Heather P.
Bugrysheva, Julia V.
Conley, Andrew B.
Gulvik, Christopher A.
Cherney, Blake
Kolton, Cari B.
Marston, Chung K.
Saile, Elke
Swaney, Erin
Lonsway, David
Gargis, Amy S.
Kongphet-Tran, Thiphasone
Lascols, Christine
Michel, Pierre
Villanueva, Julie
Hoffmaster, Alex R.
Gee, Jay E.
Sue, David
author_sort McLaughlin, Heather P.
collection PubMed
description Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.
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spelling pubmed-69868372020-02-06 Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness McLaughlin, Heather P. Bugrysheva, Julia V. Conley, Andrew B. Gulvik, Christopher A. Cherney, Blake Kolton, Cari B. Marston, Chung K. Saile, Elke Swaney, Erin Lonsway, David Gargis, Amy S. Kongphet-Tran, Thiphasone Lascols, Christine Michel, Pierre Villanueva, Julie Hoffmaster, Alex R. Gee, Jay E. Sue, David Emerg Infect Dis Dispatch Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response. Centers for Disease Control and Prevention 2020-02 /pmc/articles/PMC6986837/ /pubmed/31961318 http://dx.doi.org/10.3201/eid2602.191351 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
McLaughlin, Heather P.
Bugrysheva, Julia V.
Conley, Andrew B.
Gulvik, Christopher A.
Cherney, Blake
Kolton, Cari B.
Marston, Chung K.
Saile, Elke
Swaney, Erin
Lonsway, David
Gargis, Amy S.
Kongphet-Tran, Thiphasone
Lascols, Christine
Michel, Pierre
Villanueva, Julie
Hoffmaster, Alex R.
Gee, Jay E.
Sue, David
Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_full Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_fullStr Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_full_unstemmed Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_short Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
title_sort rapid nanopore whole-genome sequencing for anthrax emergency preparedness
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986837/
https://www.ncbi.nlm.nih.gov/pubmed/31961318
http://dx.doi.org/10.3201/eid2602.191351
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