Cargando…
Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome
The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment. Thus, we developed SR fluor...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987131/ https://www.ncbi.nlm.nih.gov/pubmed/32025294 http://dx.doi.org/10.1038/s41377-020-0249-4 |
_version_ | 1783492082692784128 |
---|---|
author | Dong, Dashan Huang, Xiaoshuai Li, Liuju Mao, Heng Mo, Yanquan Zhang, Guangyi Zhang, Zhe Shen, Jiayu Liu, Wei Wu, Zeming Liu, Guanghui Liu, Yanmei Yang, Hong Gong, Qihuang Shi, Kebin Chen, Liangyi |
author_facet | Dong, Dashan Huang, Xiaoshuai Li, Liuju Mao, Heng Mo, Yanquan Zhang, Guangyi Zhang, Zhe Shen, Jiayu Liu, Wei Wu, Zeming Liu, Guanghui Liu, Yanmei Yang, Hong Gong, Qihuang Shi, Kebin Chen, Liangyi |
author_sort | Dong, Dashan |
collection | PubMed |
description | The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment. Thus, we developed SR fluorescence-assisted diffraction computational tomography (SR-FACT), which combines label-free three-dimensional optical diffraction tomography (ODT) with two-dimensional fluorescence Hessian structured illumination microscopy. The ODT module is capable of resolving the mitochondria, lipid droplets, the nuclear membrane, chromosomes, the tubular endoplasmic reticulum, and lysosomes. Using dual-mode correlated live-cell imaging for a prolonged period of time, we observed novel subcellular structures named dark-vacuole bodies, the majority of which originate from densely populated perinuclear regions, and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma membrane. This work demonstrates the unique capabilities of SR-FACT, which suggests its wide applicability in cell biology in general. |
format | Online Article Text |
id | pubmed-6987131 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69871312020-02-05 Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome Dong, Dashan Huang, Xiaoshuai Li, Liuju Mao, Heng Mo, Yanquan Zhang, Guangyi Zhang, Zhe Shen, Jiayu Liu, Wei Wu, Zeming Liu, Guanghui Liu, Yanmei Yang, Hong Gong, Qihuang Shi, Kebin Chen, Liangyi Light Sci Appl Article The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment. Thus, we developed SR fluorescence-assisted diffraction computational tomography (SR-FACT), which combines label-free three-dimensional optical diffraction tomography (ODT) with two-dimensional fluorescence Hessian structured illumination microscopy. The ODT module is capable of resolving the mitochondria, lipid droplets, the nuclear membrane, chromosomes, the tubular endoplasmic reticulum, and lysosomes. Using dual-mode correlated live-cell imaging for a prolonged period of time, we observed novel subcellular structures named dark-vacuole bodies, the majority of which originate from densely populated perinuclear regions, and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma membrane. This work demonstrates the unique capabilities of SR-FACT, which suggests its wide applicability in cell biology in general. Nature Publishing Group UK 2020-01-28 /pmc/articles/PMC6987131/ /pubmed/32025294 http://dx.doi.org/10.1038/s41377-020-0249-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dong, Dashan Huang, Xiaoshuai Li, Liuju Mao, Heng Mo, Yanquan Zhang, Guangyi Zhang, Zhe Shen, Jiayu Liu, Wei Wu, Zeming Liu, Guanghui Liu, Yanmei Yang, Hong Gong, Qihuang Shi, Kebin Chen, Liangyi Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title | Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title_full | Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title_fullStr | Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title_full_unstemmed | Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title_short | Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
title_sort | super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987131/ https://www.ncbi.nlm.nih.gov/pubmed/32025294 http://dx.doi.org/10.1038/s41377-020-0249-4 |
work_keys_str_mv | AT dongdashan superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT huangxiaoshuai superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT liliuju superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT maoheng superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT moyanquan superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT zhangguangyi superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT zhangzhe superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT shenjiayu superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT liuwei superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT wuzeming superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT liuguanghui superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT liuyanmei superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT yanghong superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT gongqihuang superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT shikebin superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome AT chenliangyi superresolutionfluorescenceassisteddiffractioncomputationaltomographyrevealsthethreedimensionallandscapeofthecellularorganelleinteractome |