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Molecular characterization and safety assessment of biofortified provitamin A rice
Part of the studies involved in safety assessment of genetically engineered crops includes characterizing the organization, integrity, and stability of the inserted DNA and evaluating the potential allergenicity and toxicity of newly-expressed proteins. Molecular characterization of the introduced D...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987151/ https://www.ncbi.nlm.nih.gov/pubmed/31992721 http://dx.doi.org/10.1038/s41598-020-57669-5 |
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author | Oliva, Norman Florida Cueto-Reaño, Maria Trijatmiko, Kurniawan R. Samia, Mercy Welsch, Ralf Schaub, Patrick Beyer, Peter Mackenzie, Donald Boncodin, Raul Reinke, Russell Slamet-Loedin, Inez Mallikarjuna Swamy, B. P. |
author_facet | Oliva, Norman Florida Cueto-Reaño, Maria Trijatmiko, Kurniawan R. Samia, Mercy Welsch, Ralf Schaub, Patrick Beyer, Peter Mackenzie, Donald Boncodin, Raul Reinke, Russell Slamet-Loedin, Inez Mallikarjuna Swamy, B. P. |
author_sort | Oliva, Norman |
collection | PubMed |
description | Part of the studies involved in safety assessment of genetically engineered crops includes characterizing the organization, integrity, and stability of the inserted DNA and evaluating the potential allergenicity and toxicity of newly-expressed proteins. Molecular characterization of the introduced DNA in provitamin A biofortified rice event GR2E confirmed insertion of a single copy of the transfer-DNA in the genome and its inheritance as a single locus. Nucleotide sequencing of the inserted DNA confirmed it was introduced without modifications. The phytoene synthase, and carotene desaturase proteins did not display sequence similarity with allergens or toxins. Both proteins were rapidly digested in simulated gastric fluid and their enzymatic activity was inhibited upon heat treatment. Acute oral toxicity testing of the protein in mice demonstrated lack of adverse effects. These evidences substantiated the lack of any identifiable hazards for both proteins and in combination with other existing comparative analyses provided assurance that food derived from this rice is safe. This conclusion is in line with those of the regulatory agencies of US Food and Drug Administration, Health Canada and Food Standard Australia and New Zealand. |
format | Online Article Text |
id | pubmed-6987151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69871512020-02-03 Molecular characterization and safety assessment of biofortified provitamin A rice Oliva, Norman Florida Cueto-Reaño, Maria Trijatmiko, Kurniawan R. Samia, Mercy Welsch, Ralf Schaub, Patrick Beyer, Peter Mackenzie, Donald Boncodin, Raul Reinke, Russell Slamet-Loedin, Inez Mallikarjuna Swamy, B. P. Sci Rep Article Part of the studies involved in safety assessment of genetically engineered crops includes characterizing the organization, integrity, and stability of the inserted DNA and evaluating the potential allergenicity and toxicity of newly-expressed proteins. Molecular characterization of the introduced DNA in provitamin A biofortified rice event GR2E confirmed insertion of a single copy of the transfer-DNA in the genome and its inheritance as a single locus. Nucleotide sequencing of the inserted DNA confirmed it was introduced without modifications. The phytoene synthase, and carotene desaturase proteins did not display sequence similarity with allergens or toxins. Both proteins were rapidly digested in simulated gastric fluid and their enzymatic activity was inhibited upon heat treatment. Acute oral toxicity testing of the protein in mice demonstrated lack of adverse effects. These evidences substantiated the lack of any identifiable hazards for both proteins and in combination with other existing comparative analyses provided assurance that food derived from this rice is safe. This conclusion is in line with those of the regulatory agencies of US Food and Drug Administration, Health Canada and Food Standard Australia and New Zealand. Nature Publishing Group UK 2020-01-28 /pmc/articles/PMC6987151/ /pubmed/31992721 http://dx.doi.org/10.1038/s41598-020-57669-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Oliva, Norman Florida Cueto-Reaño, Maria Trijatmiko, Kurniawan R. Samia, Mercy Welsch, Ralf Schaub, Patrick Beyer, Peter Mackenzie, Donald Boncodin, Raul Reinke, Russell Slamet-Loedin, Inez Mallikarjuna Swamy, B. P. Molecular characterization and safety assessment of biofortified provitamin A rice |
title | Molecular characterization and safety assessment of biofortified provitamin A rice |
title_full | Molecular characterization and safety assessment of biofortified provitamin A rice |
title_fullStr | Molecular characterization and safety assessment of biofortified provitamin A rice |
title_full_unstemmed | Molecular characterization and safety assessment of biofortified provitamin A rice |
title_short | Molecular characterization and safety assessment of biofortified provitamin A rice |
title_sort | molecular characterization and safety assessment of biofortified provitamin a rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987151/ https://www.ncbi.nlm.nih.gov/pubmed/31992721 http://dx.doi.org/10.1038/s41598-020-57669-5 |
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