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Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia

BACKGROUND: To reveal trends in bone microarchitectural parameters with increasing spatial resolution on ultra-high-resolution computed tomography (UHRCT) in vivo and to compare its performance with that of conventional-resolution CT (CRCT) and micro-CT ex vivo. METHODS: We retrospectively assessed...

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Autores principales: Inai, Ryota, Nakahara, Ryuichi, Morimitsu, Yusuke, Akagi, Noriaki, Marukawa, Youhei, Matsushita, Toshi, Tanaka, Takashi, Tada, Akihiro, Hiraki, Takao, Nasu, Yoshihisa, Nishida, Keiichiro, Ozaki, Toshifumi, Kanazawa, Susumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987291/
https://www.ncbi.nlm.nih.gov/pubmed/31993864
http://dx.doi.org/10.1186/s41747-019-0135-0
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author Inai, Ryota
Nakahara, Ryuichi
Morimitsu, Yusuke
Akagi, Noriaki
Marukawa, Youhei
Matsushita, Toshi
Tanaka, Takashi
Tada, Akihiro
Hiraki, Takao
Nasu, Yoshihisa
Nishida, Keiichiro
Ozaki, Toshifumi
Kanazawa, Susumu
author_facet Inai, Ryota
Nakahara, Ryuichi
Morimitsu, Yusuke
Akagi, Noriaki
Marukawa, Youhei
Matsushita, Toshi
Tanaka, Takashi
Tada, Akihiro
Hiraki, Takao
Nasu, Yoshihisa
Nishida, Keiichiro
Ozaki, Toshifumi
Kanazawa, Susumu
author_sort Inai, Ryota
collection PubMed
description BACKGROUND: To reveal trends in bone microarchitectural parameters with increasing spatial resolution on ultra-high-resolution computed tomography (UHRCT) in vivo and to compare its performance with that of conventional-resolution CT (CRCT) and micro-CT ex vivo. METHODS: We retrospectively assessed 5 tiger vertebrae ex vivo and 16 human tibiae in vivo. Seven-pattern and four-pattern resolution imaging were performed on tiger vertebra using CRCT, UHRCT, and micro-CT, and on human tibiae using UHRCT. We measured six microarchitectural parameters: volumetric bone mineral density (vBMD), trabecular bone volume fraction (bone volume/total volume, BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and connectivity density (ConnD). Comparisons between different imaging resolutions were performed using Tukey or Dunnett T3 test. RESULTS: The vBMD, BV/TV, Tb.N, and ConnD parameters showed an increasing trend, while Tb.Sp showed a decreasing trend both ex vivo and in vivo. Ex vivo, UHRCT at the two highest resolutions (1024- and 2048-matrix imaging with 0.25-mm slice thickness) and CRCT showed significant differences (p ≤ 0.047) in vBMD (51.4 mg/cm(3) and 63.5 mg/cm(3) versus 20.8 mg/cm(3)), BV/TV (26.5% and 29.5% versus 13.8 %), Tb.N (1.3 l/mm and 1.48 l/mm versus 0.47 l/mm), and ConnD (0.52 l/mm(3) and 0.74 l/mm(3) versus 0.02 l/mm(3), respectively). In vivo, the 512- and 1024-matrix imaging with 0.25-mm slice thickness showed significant differences in Tb.N (0.38 l/mm versus 0.67 l/mm, respectively) and ConnD (0.06 l/mm(3) versus 0.22 l/mm(3), respectively). CONCLUSIONS: We observed characteristic trends in microarchitectural parameters and demonstrated the potential utility of applying UHRCT for microarchitectural analysis.
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spelling pubmed-69872912020-02-11 Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia Inai, Ryota Nakahara, Ryuichi Morimitsu, Yusuke Akagi, Noriaki Marukawa, Youhei Matsushita, Toshi Tanaka, Takashi Tada, Akihiro Hiraki, Takao Nasu, Yoshihisa Nishida, Keiichiro Ozaki, Toshifumi Kanazawa, Susumu Eur Radiol Exp Original Article BACKGROUND: To reveal trends in bone microarchitectural parameters with increasing spatial resolution on ultra-high-resolution computed tomography (UHRCT) in vivo and to compare its performance with that of conventional-resolution CT (CRCT) and micro-CT ex vivo. METHODS: We retrospectively assessed 5 tiger vertebrae ex vivo and 16 human tibiae in vivo. Seven-pattern and four-pattern resolution imaging were performed on tiger vertebra using CRCT, UHRCT, and micro-CT, and on human tibiae using UHRCT. We measured six microarchitectural parameters: volumetric bone mineral density (vBMD), trabecular bone volume fraction (bone volume/total volume, BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and connectivity density (ConnD). Comparisons between different imaging resolutions were performed using Tukey or Dunnett T3 test. RESULTS: The vBMD, BV/TV, Tb.N, and ConnD parameters showed an increasing trend, while Tb.Sp showed a decreasing trend both ex vivo and in vivo. Ex vivo, UHRCT at the two highest resolutions (1024- and 2048-matrix imaging with 0.25-mm slice thickness) and CRCT showed significant differences (p ≤ 0.047) in vBMD (51.4 mg/cm(3) and 63.5 mg/cm(3) versus 20.8 mg/cm(3)), BV/TV (26.5% and 29.5% versus 13.8 %), Tb.N (1.3 l/mm and 1.48 l/mm versus 0.47 l/mm), and ConnD (0.52 l/mm(3) and 0.74 l/mm(3) versus 0.02 l/mm(3), respectively). In vivo, the 512- and 1024-matrix imaging with 0.25-mm slice thickness showed significant differences in Tb.N (0.38 l/mm versus 0.67 l/mm, respectively) and ConnD (0.06 l/mm(3) versus 0.22 l/mm(3), respectively). CONCLUSIONS: We observed characteristic trends in microarchitectural parameters and demonstrated the potential utility of applying UHRCT for microarchitectural analysis. Springer International Publishing 2020-01-28 /pmc/articles/PMC6987291/ /pubmed/31993864 http://dx.doi.org/10.1186/s41747-019-0135-0 Text en © The Author(s) 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Inai, Ryota
Nakahara, Ryuichi
Morimitsu, Yusuke
Akagi, Noriaki
Marukawa, Youhei
Matsushita, Toshi
Tanaka, Takashi
Tada, Akihiro
Hiraki, Takao
Nasu, Yoshihisa
Nishida, Keiichiro
Ozaki, Toshifumi
Kanazawa, Susumu
Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title_full Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title_fullStr Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title_full_unstemmed Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title_short Bone microarchitectural analysis using ultra-high-resolution CT in tiger vertebra and human tibia
title_sort bone microarchitectural analysis using ultra-high-resolution ct in tiger vertebra and human tibia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987291/
https://www.ncbi.nlm.nih.gov/pubmed/31993864
http://dx.doi.org/10.1186/s41747-019-0135-0
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