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Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA

BACKGROUND: Marek’s disease (MD) is caused by the oncogenic Marek’s disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The...

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Autores principales: Ding, Ke, Yu, Zu-Hua, Yu, Chuan, Jia, Yan-Yan, He, Lei, Liao, Cheng-Shui, Li, Jing, Zhang, Chun-Jie, Li, Yin-Ju, Wu, Ting-Cai, Cheng, Xiang-Chao, Zhou, Zi-Yu, Yu, Zu-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988224/
https://www.ncbi.nlm.nih.gov/pubmed/31992293
http://dx.doi.org/10.1186/s12917-020-2239-4
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author Ding, Ke
Yu, Zu-Hua
Yu, Chuan
Jia, Yan-Yan
He, Lei
Liao, Cheng-Shui
Li, Jing
Zhang, Chun-Jie
Li, Yin-Ju
Wu, Ting-Cai
Cheng, Xiang-Chao
Zhou, Zi-Yu
Yu, Zu-Ling
author_facet Ding, Ke
Yu, Zu-Hua
Yu, Chuan
Jia, Yan-Yan
He, Lei
Liao, Cheng-Shui
Li, Jing
Zhang, Chun-Jie
Li, Yin-Ju
Wu, Ting-Cai
Cheng, Xiang-Chao
Zhou, Zi-Yu
Yu, Zu-Ling
author_sort Ding, Ke
collection PubMed
description BACKGROUND: Marek’s disease (MD) is caused by the oncogenic Marek’s disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. RESULTS: The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. CONCLUSION: The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.
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spelling pubmed-69882242020-01-31 Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA Ding, Ke Yu, Zu-Hua Yu, Chuan Jia, Yan-Yan He, Lei Liao, Cheng-Shui Li, Jing Zhang, Chun-Jie Li, Yin-Ju Wu, Ting-Cai Cheng, Xiang-Chao Zhou, Zi-Yu Yu, Zu-Ling BMC Vet Res Research Article BACKGROUND: Marek’s disease (MD) is caused by the oncogenic Marek’s disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. RESULTS: The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. CONCLUSION: The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene. BioMed Central 2020-01-28 /pmc/articles/PMC6988224/ /pubmed/31992293 http://dx.doi.org/10.1186/s12917-020-2239-4 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ding, Ke
Yu, Zu-Hua
Yu, Chuan
Jia, Yan-Yan
He, Lei
Liao, Cheng-Shui
Li, Jing
Zhang, Chun-Jie
Li, Yin-Ju
Wu, Ting-Cai
Cheng, Xiang-Chao
Zhou, Zi-Yu
Yu, Zu-Ling
Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title_full Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title_fullStr Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title_full_unstemmed Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title_short Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA
title_sort effect of gga-mir-155 on cell proliferation, apoptosis and invasion of marek’s disease virus (mdv) transformed cell line msb1 by targeting rora
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988224/
https://www.ncbi.nlm.nih.gov/pubmed/31992293
http://dx.doi.org/10.1186/s12917-020-2239-4
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