Pre-emptive genomic surveillance of emerging ebolaviruses

Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing g...

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Detalles Bibliográficos
Autores principales: Postigo-Hidalgo, Ignacio, Fischer, Carlo, Moreira-Soto, Andres, Tscheak, Patricia, Nagel, Michael, Eickmann, Markus, Drexler, Jan Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988270/
https://www.ncbi.nlm.nih.gov/pubmed/31992392
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.3.1900765
Descripción
Sumario:Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.

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