Pre-emptive genomic surveillance of emerging ebolaviruses

Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing g...

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Autores principales: Postigo-Hidalgo, Ignacio, Fischer, Carlo, Moreira-Soto, Andres, Tscheak, Patricia, Nagel, Michael, Eickmann, Markus, Drexler, Jan Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988270/
https://www.ncbi.nlm.nih.gov/pubmed/31992392
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.3.1900765
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author Postigo-Hidalgo, Ignacio
Fischer, Carlo
Moreira-Soto, Andres
Tscheak, Patricia
Nagel, Michael
Eickmann, Markus
Drexler, Jan Felix
author_facet Postigo-Hidalgo, Ignacio
Fischer, Carlo
Moreira-Soto, Andres
Tscheak, Patricia
Nagel, Michael
Eickmann, Markus
Drexler, Jan Felix
author_sort Postigo-Hidalgo, Ignacio
collection PubMed
description Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.
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spelling pubmed-69882702020-02-06 Pre-emptive genomic surveillance of emerging ebolaviruses Postigo-Hidalgo, Ignacio Fischer, Carlo Moreira-Soto, Andres Tscheak, Patricia Nagel, Michael Eickmann, Markus Drexler, Jan Felix Euro Surveill Rapid Communication Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence. European Centre for Disease Prevention and Control (ECDC) 2020-01-23 /pmc/articles/PMC6988270/ /pubmed/31992392 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.3.1900765 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Postigo-Hidalgo, Ignacio
Fischer, Carlo
Moreira-Soto, Andres
Tscheak, Patricia
Nagel, Michael
Eickmann, Markus
Drexler, Jan Felix
Pre-emptive genomic surveillance of emerging ebolaviruses
title Pre-emptive genomic surveillance of emerging ebolaviruses
title_full Pre-emptive genomic surveillance of emerging ebolaviruses
title_fullStr Pre-emptive genomic surveillance of emerging ebolaviruses
title_full_unstemmed Pre-emptive genomic surveillance of emerging ebolaviruses
title_short Pre-emptive genomic surveillance of emerging ebolaviruses
title_sort pre-emptive genomic surveillance of emerging ebolaviruses
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988270/
https://www.ncbi.nlm.nih.gov/pubmed/31992392
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.3.1900765
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