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Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco

Cucumber mosaic virus (CMV), which is vectored by aphids, has a tripartite RNA genome encoding five proteins. In tobacco (Nicotiana tabacum), a subgroup IA CMV strain, Fny‐CMV, increases plant susceptibility to aphid infestation but a viral mutant unable to express the 2b protein (Fny‐CMV∆2b) induce...

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Autores principales: Tungadi, Trisna, Donnelly, Ruairí, Qing, Ling, Iqbal, Javaid, Murphy, Alex M., Pate, Adrienne E., Cunniffe, Nik J., Carr, John P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988427/
https://www.ncbi.nlm.nih.gov/pubmed/31777194
http://dx.doi.org/10.1111/mpp.12892
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author Tungadi, Trisna
Donnelly, Ruairí
Qing, Ling
Iqbal, Javaid
Murphy, Alex M.
Pate, Adrienne E.
Cunniffe, Nik J.
Carr, John P.
author_facet Tungadi, Trisna
Donnelly, Ruairí
Qing, Ling
Iqbal, Javaid
Murphy, Alex M.
Pate, Adrienne E.
Cunniffe, Nik J.
Carr, John P.
author_sort Tungadi, Trisna
collection PubMed
description Cucumber mosaic virus (CMV), which is vectored by aphids, has a tripartite RNA genome encoding five proteins. In tobacco (Nicotiana tabacum), a subgroup IA CMV strain, Fny‐CMV, increases plant susceptibility to aphid infestation but a viral mutant unable to express the 2b protein (Fny‐CMV∆2b) induces aphid resistance. We hypothesized that in tobacco, one or more of the four other Fny‐CMV gene products (the 1a or 2a replication proteins, the movement protein, or the coat protein) are potential aphid resistance elicitors, whilst the 2b protein counteracts induction of aphid resistance. Mutation of the Fny‐CMV 2b protein indicated that inhibition of virus‐induced resistance to aphids (Myzus persicae) depends on amino acid sequences known to control nucleus‐to‐cytoplasm shuttling. LS‐CMV (subgroup II) also increased susceptibility to aphid infestation but the LS‐CMV∆2b mutant did not induce aphid resistance. Using reassortant viruses comprising different combinations of LS and Fny genomic RNAs, we showed that Fny‐CMV RNA 1 but not LS‐CMV RNA 1 conditions aphid resistance in tobacco, suggesting that the Fny‐CMV 1a protein triggers resistance. However, the 2b proteins of both strains suppress aphid resistance, suggesting that the ability of 2b proteins to inhibit aphid resistance is conserved among divergent CMV strains.
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spelling pubmed-69884272020-02-03 Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco Tungadi, Trisna Donnelly, Ruairí Qing, Ling Iqbal, Javaid Murphy, Alex M. Pate, Adrienne E. Cunniffe, Nik J. Carr, John P. Mol Plant Pathol Short Communications Cucumber mosaic virus (CMV), which is vectored by aphids, has a tripartite RNA genome encoding five proteins. In tobacco (Nicotiana tabacum), a subgroup IA CMV strain, Fny‐CMV, increases plant susceptibility to aphid infestation but a viral mutant unable to express the 2b protein (Fny‐CMV∆2b) induces aphid resistance. We hypothesized that in tobacco, one or more of the four other Fny‐CMV gene products (the 1a or 2a replication proteins, the movement protein, or the coat protein) are potential aphid resistance elicitors, whilst the 2b protein counteracts induction of aphid resistance. Mutation of the Fny‐CMV 2b protein indicated that inhibition of virus‐induced resistance to aphids (Myzus persicae) depends on amino acid sequences known to control nucleus‐to‐cytoplasm shuttling. LS‐CMV (subgroup II) also increased susceptibility to aphid infestation but the LS‐CMV∆2b mutant did not induce aphid resistance. Using reassortant viruses comprising different combinations of LS and Fny genomic RNAs, we showed that Fny‐CMV RNA 1 but not LS‐CMV RNA 1 conditions aphid resistance in tobacco, suggesting that the Fny‐CMV 1a protein triggers resistance. However, the 2b proteins of both strains suppress aphid resistance, suggesting that the ability of 2b proteins to inhibit aphid resistance is conserved among divergent CMV strains. John Wiley and Sons Inc. 2019-11-27 /pmc/articles/PMC6988427/ /pubmed/31777194 http://dx.doi.org/10.1111/mpp.12892 Text en © 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Tungadi, Trisna
Donnelly, Ruairí
Qing, Ling
Iqbal, Javaid
Murphy, Alex M.
Pate, Adrienne E.
Cunniffe, Nik J.
Carr, John P.
Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title_full Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title_fullStr Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title_full_unstemmed Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title_short Cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
title_sort cucumber mosaic virus 2b proteins inhibit virus‐induced aphid resistance in tobacco
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988427/
https://www.ncbi.nlm.nih.gov/pubmed/31777194
http://dx.doi.org/10.1111/mpp.12892
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