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Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants

Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce spec...

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Autores principales: Raya Tonetti, Fernanda, Arce, Lorena, Salva, Susana, Alvarez, Susana, Takahashi, Hideki, Kitazawa, Haruki, Vizoso-Pinto, Maria Guadalupe, Villena, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989447/
https://www.ncbi.nlm.nih.gov/pubmed/32038659
http://dx.doi.org/10.3389/fimmu.2020.00015
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author Raya Tonetti, Fernanda
Arce, Lorena
Salva, Susana
Alvarez, Susana
Takahashi, Hideki
Kitazawa, Haruki
Vizoso-Pinto, Maria Guadalupe
Villena, Julio
author_facet Raya Tonetti, Fernanda
Arce, Lorena
Salva, Susana
Alvarez, Susana
Takahashi, Hideki
Kitazawa, Haruki
Vizoso-Pinto, Maria Guadalupe
Villena, Julio
author_sort Raya Tonetti, Fernanda
collection PubMed
description Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce specific immune responses. It is well-established that the immunomodulatory properties of LAB are strain dependent and therefore, the BLPs derived from each individual strain could have different adjuvant capacities. In this work, we obtained BLPs from immunomodulatory (immunobiotics) and non-immunomodulatory Lactobacillus rhamnosus and Lactobacillus plantarum strains and comparatively evaluated their ability to improve the intestinal and systemic immune responses elicited by an attenuated rotavirus vaccine. Results demonstrated that orally administered BLPs from non-immunomodulatory strains did not induce significant changes in the immune response triggered by rotavirus vaccine in mice. On the contrary, BLPs derived from immunobiotic lactobacilli were able to improve the levels of anti-rotavirus intestinal IgA and serum IgG, the numbers of CD24(+)B220(+) B and CD4(+) T cells in Peyer's patches and spleen as well as the production of IFN-γ by immune cells. Interestingly, among immunobiotics-derived BLPs, those obtained from L. rhamnosus CRL1505 and L. rhamnosus IBL027 enhanced more efficiently the intestinal and systemic humoral immune responses when compared to BLPs from other immunobiotic bacteria. The findings of this work indicate that it is necessary to perform an appropriate selection of BLPs in order to find those with the most efficient adjuvant properties. We propose the term Immunobiotic-like particles (IBLPs) for the BLPs derived from CRL1505 and IBL027 strains that are an excellent alternative for the development of mucosal vaccines.
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spelling pubmed-69894472020-02-07 Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants Raya Tonetti, Fernanda Arce, Lorena Salva, Susana Alvarez, Susana Takahashi, Hideki Kitazawa, Haruki Vizoso-Pinto, Maria Guadalupe Villena, Julio Front Immunol Immunology Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce specific immune responses. It is well-established that the immunomodulatory properties of LAB are strain dependent and therefore, the BLPs derived from each individual strain could have different adjuvant capacities. In this work, we obtained BLPs from immunomodulatory (immunobiotics) and non-immunomodulatory Lactobacillus rhamnosus and Lactobacillus plantarum strains and comparatively evaluated their ability to improve the intestinal and systemic immune responses elicited by an attenuated rotavirus vaccine. Results demonstrated that orally administered BLPs from non-immunomodulatory strains did not induce significant changes in the immune response triggered by rotavirus vaccine in mice. On the contrary, BLPs derived from immunobiotic lactobacilli were able to improve the levels of anti-rotavirus intestinal IgA and serum IgG, the numbers of CD24(+)B220(+) B and CD4(+) T cells in Peyer's patches and spleen as well as the production of IFN-γ by immune cells. Interestingly, among immunobiotics-derived BLPs, those obtained from L. rhamnosus CRL1505 and L. rhamnosus IBL027 enhanced more efficiently the intestinal and systemic humoral immune responses when compared to BLPs from other immunobiotic bacteria. The findings of this work indicate that it is necessary to perform an appropriate selection of BLPs in order to find those with the most efficient adjuvant properties. We propose the term Immunobiotic-like particles (IBLPs) for the BLPs derived from CRL1505 and IBL027 strains that are an excellent alternative for the development of mucosal vaccines. Frontiers Media S.A. 2020-01-23 /pmc/articles/PMC6989447/ /pubmed/32038659 http://dx.doi.org/10.3389/fimmu.2020.00015 Text en Copyright © 2020 Raya Tonetti, Arce, Salva, Alvarez, Takahashi, Kitazawa, Vizoso-Pinto and Villena. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Raya Tonetti, Fernanda
Arce, Lorena
Salva, Susana
Alvarez, Susana
Takahashi, Hideki
Kitazawa, Haruki
Vizoso-Pinto, Maria Guadalupe
Villena, Julio
Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title_full Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title_fullStr Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title_full_unstemmed Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title_short Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants
title_sort immunomodulatory properties of bacterium-like particles obtained from immunobiotic lactobacilli: prospects for their use as mucosal adjuvants
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989447/
https://www.ncbi.nlm.nih.gov/pubmed/32038659
http://dx.doi.org/10.3389/fimmu.2020.00015
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