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Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivatio...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989686/ https://www.ncbi.nlm.nih.gov/pubmed/31996685 http://dx.doi.org/10.1038/s41467-019-14176-0 |
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author | Kong, Jinuk Ji, Yul Jeon, Yong Geun Han, Ji Seul Han, Kyung Hee Lee, Jung Hyun Lee, Gung Jang, Hagoon Choe, Sung Sik Baes, Myriam Kim, Jae Bum |
author_facet | Kong, Jinuk Ji, Yul Jeon, Yong Geun Han, Ji Seul Han, Kyung Hee Lee, Jung Hyun Lee, Gung Jang, Hagoon Choe, Sung Sik Baes, Myriam Kim, Jae Bum |
author_sort | Kong, Jinuk |
collection | PubMed |
description | Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivation. Here, we demonstrate that peroxisomes and peroxisomal protein PEX5 mediate fasting-induced lipolysis by stimulating adipose triglyceride lipase (ATGL) translocation onto LDs. During fasting, physical contacts between peroxisomes and LDs are increased by KIFC3-dependent movement of peroxisomes toward LDs, which facilitates spatial translocations of ATGL onto LDs. In addition, PEX5 could escort ATGL to contact points between peroxisomes and LDs in the presence of fasting cues. Moreover, in adipocyte-specific PEX5-knockout mice, the recruitment of ATGL onto LDs was defective and fasting-induced lipolysis is attenuated. Collectively, these data suggest that physical contacts between peroxisomes and LDs are required for spatiotemporal translocation of ATGL, which is escorted by PEX5 upon fasting, to maintain energy homeostasis. |
format | Online Article Text |
id | pubmed-6989686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69896862020-01-31 Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 Kong, Jinuk Ji, Yul Jeon, Yong Geun Han, Ji Seul Han, Kyung Hee Lee, Jung Hyun Lee, Gung Jang, Hagoon Choe, Sung Sik Baes, Myriam Kim, Jae Bum Nat Commun Article Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivation. Here, we demonstrate that peroxisomes and peroxisomal protein PEX5 mediate fasting-induced lipolysis by stimulating adipose triglyceride lipase (ATGL) translocation onto LDs. During fasting, physical contacts between peroxisomes and LDs are increased by KIFC3-dependent movement of peroxisomes toward LDs, which facilitates spatial translocations of ATGL onto LDs. In addition, PEX5 could escort ATGL to contact points between peroxisomes and LDs in the presence of fasting cues. Moreover, in adipocyte-specific PEX5-knockout mice, the recruitment of ATGL onto LDs was defective and fasting-induced lipolysis is attenuated. Collectively, these data suggest that physical contacts between peroxisomes and LDs are required for spatiotemporal translocation of ATGL, which is escorted by PEX5 upon fasting, to maintain energy homeostasis. Nature Publishing Group UK 2020-01-29 /pmc/articles/PMC6989686/ /pubmed/31996685 http://dx.doi.org/10.1038/s41467-019-14176-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kong, Jinuk Ji, Yul Jeon, Yong Geun Han, Ji Seul Han, Kyung Hee Lee, Jung Hyun Lee, Gung Jang, Hagoon Choe, Sung Sik Baes, Myriam Kim, Jae Bum Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title | Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title_full | Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title_fullStr | Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title_full_unstemmed | Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title_short | Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 |
title_sort | spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via pex5 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989686/ https://www.ncbi.nlm.nih.gov/pubmed/31996685 http://dx.doi.org/10.1038/s41467-019-14176-0 |
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