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Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5

Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivatio...

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Autores principales: Kong, Jinuk, Ji, Yul, Jeon, Yong Geun, Han, Ji Seul, Han, Kyung Hee, Lee, Jung Hyun, Lee, Gung, Jang, Hagoon, Choe, Sung Sik, Baes, Myriam, Kim, Jae Bum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989686/
https://www.ncbi.nlm.nih.gov/pubmed/31996685
http://dx.doi.org/10.1038/s41467-019-14176-0
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author Kong, Jinuk
Ji, Yul
Jeon, Yong Geun
Han, Ji Seul
Han, Kyung Hee
Lee, Jung Hyun
Lee, Gung
Jang, Hagoon
Choe, Sung Sik
Baes, Myriam
Kim, Jae Bum
author_facet Kong, Jinuk
Ji, Yul
Jeon, Yong Geun
Han, Ji Seul
Han, Kyung Hee
Lee, Jung Hyun
Lee, Gung
Jang, Hagoon
Choe, Sung Sik
Baes, Myriam
Kim, Jae Bum
author_sort Kong, Jinuk
collection PubMed
description Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivation. Here, we demonstrate that peroxisomes and peroxisomal protein PEX5 mediate fasting-induced lipolysis by stimulating adipose triglyceride lipase (ATGL) translocation onto LDs. During fasting, physical contacts between peroxisomes and LDs are increased by KIFC3-dependent movement of peroxisomes toward LDs, which facilitates spatial translocations of ATGL onto LDs. In addition, PEX5 could escort ATGL to contact points between peroxisomes and LDs in the presence of fasting cues. Moreover, in adipocyte-specific PEX5-knockout mice, the recruitment of ATGL onto LDs was defective and fasting-induced lipolysis is attenuated. Collectively, these data suggest that physical contacts between peroxisomes and LDs are required for spatiotemporal translocation of ATGL, which is escorted by PEX5 upon fasting, to maintain energy homeostasis.
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spelling pubmed-69896862020-01-31 Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5 Kong, Jinuk Ji, Yul Jeon, Yong Geun Han, Ji Seul Han, Kyung Hee Lee, Jung Hyun Lee, Gung Jang, Hagoon Choe, Sung Sik Baes, Myriam Kim, Jae Bum Nat Commun Article Lipid droplets (LDs) are key subcellular organelles for regulating lipid metabolism. Although several subcellular organelles participate in lipid metabolism, it remains elusive whether physical contacts between subcellular organelles and LDs might be involved in lipolysis upon nutritional deprivation. Here, we demonstrate that peroxisomes and peroxisomal protein PEX5 mediate fasting-induced lipolysis by stimulating adipose triglyceride lipase (ATGL) translocation onto LDs. During fasting, physical contacts between peroxisomes and LDs are increased by KIFC3-dependent movement of peroxisomes toward LDs, which facilitates spatial translocations of ATGL onto LDs. In addition, PEX5 could escort ATGL to contact points between peroxisomes and LDs in the presence of fasting cues. Moreover, in adipocyte-specific PEX5-knockout mice, the recruitment of ATGL onto LDs was defective and fasting-induced lipolysis is attenuated. Collectively, these data suggest that physical contacts between peroxisomes and LDs are required for spatiotemporal translocation of ATGL, which is escorted by PEX5 upon fasting, to maintain energy homeostasis. Nature Publishing Group UK 2020-01-29 /pmc/articles/PMC6989686/ /pubmed/31996685 http://dx.doi.org/10.1038/s41467-019-14176-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kong, Jinuk
Ji, Yul
Jeon, Yong Geun
Han, Ji Seul
Han, Kyung Hee
Lee, Jung Hyun
Lee, Gung
Jang, Hagoon
Choe, Sung Sik
Baes, Myriam
Kim, Jae Bum
Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title_full Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title_fullStr Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title_full_unstemmed Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title_short Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5
title_sort spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via pex5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989686/
https://www.ncbi.nlm.nih.gov/pubmed/31996685
http://dx.doi.org/10.1038/s41467-019-14176-0
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