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The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota

Poultry is a major reservoir for the pathogen Campylobacter jejuni. C. jejuni inhabits the poultry gastrointestinal tract as a part of the gut microbiota. The objective of this study was to evaluate both the survival of C. jejuni and the changes in the population dynamics of the cecal microbiome dur...

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Autores principales: Feye, Kristina M., Rubinelli, Peter M., Chaney, William Evan, Pavlidis, Hilary O., Kogut, Michael H., Ricke, Steven C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990144/
https://www.ncbi.nlm.nih.gov/pubmed/32038534
http://dx.doi.org/10.3389/fmicb.2019.03062
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author Feye, Kristina M.
Rubinelli, Peter M.
Chaney, William Evan
Pavlidis, Hilary O.
Kogut, Michael H.
Ricke, Steven C.
author_facet Feye, Kristina M.
Rubinelli, Peter M.
Chaney, William Evan
Pavlidis, Hilary O.
Kogut, Michael H.
Ricke, Steven C.
author_sort Feye, Kristina M.
collection PubMed
description Poultry is a major reservoir for the pathogen Campylobacter jejuni. C. jejuni inhabits the poultry gastrointestinal tract as a part of the gut microbiota. The objective of this study was to evaluate both the survival of C. jejuni and the changes in the population dynamics of the cecal microbiome during an in vitro C. jejuni inoculation in the presence or absence of the functional metabolites of Diamond V Original XPC(TM) (XPC). Two independent trials were conducted. Broiler chickens (n = 6 per Trial 1 and n = 3 per Trial 2) were raised according to standard industry guidelines and euthanized on Day 41. The ceca were collected aseptically, their contents removed independently and then used in an in vitro microaerobic model with 0.1% cecal contents + Campylobacter with or without 1% XPC (w/v). Before the inoculation with a chloramphenicol resistant marker strain of C. jejuni, the cecal contents were pre-incubated with XPC at 42°C for 24 h, in a shaking incubator (200 rpm) under microaerobic conditions, then experimentally inoculated with 10(8)/ml of C. jejuni into the appropriate treatment groups. At 0 and 24 h for Trial 1, and 48 h for Trial 2, sub-samples of the culture (n = 3 ceca, two technical replicates per ceca, XPC alone or ceca culture alone) were enumerated using a Petroff–Hausser counter, and the DNA was extracted for microbiome analysis. DNA was isolated using the Qiagen QIAamp Fast Stool DNA Mini Kit and sequenced using the Illumina MiSeq platform. The reads were filtered, normalized, and assigned taxonomical identities using the QIIME2 pipeline. The relative microbiota populations were identified via ANCOM. Altogether, evidence suggests that XPC alters the microbiome, and in turn reduces Campylobacter survival.
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spelling pubmed-69901442020-02-07 The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota Feye, Kristina M. Rubinelli, Peter M. Chaney, William Evan Pavlidis, Hilary O. Kogut, Michael H. Ricke, Steven C. Front Microbiol Microbiology Poultry is a major reservoir for the pathogen Campylobacter jejuni. C. jejuni inhabits the poultry gastrointestinal tract as a part of the gut microbiota. The objective of this study was to evaluate both the survival of C. jejuni and the changes in the population dynamics of the cecal microbiome during an in vitro C. jejuni inoculation in the presence or absence of the functional metabolites of Diamond V Original XPC(TM) (XPC). Two independent trials were conducted. Broiler chickens (n = 6 per Trial 1 and n = 3 per Trial 2) were raised according to standard industry guidelines and euthanized on Day 41. The ceca were collected aseptically, their contents removed independently and then used in an in vitro microaerobic model with 0.1% cecal contents + Campylobacter with or without 1% XPC (w/v). Before the inoculation with a chloramphenicol resistant marker strain of C. jejuni, the cecal contents were pre-incubated with XPC at 42°C for 24 h, in a shaking incubator (200 rpm) under microaerobic conditions, then experimentally inoculated with 10(8)/ml of C. jejuni into the appropriate treatment groups. At 0 and 24 h for Trial 1, and 48 h for Trial 2, sub-samples of the culture (n = 3 ceca, two technical replicates per ceca, XPC alone or ceca culture alone) were enumerated using a Petroff–Hausser counter, and the DNA was extracted for microbiome analysis. DNA was isolated using the Qiagen QIAamp Fast Stool DNA Mini Kit and sequenced using the Illumina MiSeq platform. The reads were filtered, normalized, and assigned taxonomical identities using the QIIME2 pipeline. The relative microbiota populations were identified via ANCOM. Altogether, evidence suggests that XPC alters the microbiome, and in turn reduces Campylobacter survival. Frontiers Media S.A. 2020-01-23 /pmc/articles/PMC6990144/ /pubmed/32038534 http://dx.doi.org/10.3389/fmicb.2019.03062 Text en Copyright © 2020 Feye, Rubinelli, Chaney, Pavlidis, Kogut and Ricke. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Feye, Kristina M.
Rubinelli, Peter M.
Chaney, William Evan
Pavlidis, Hilary O.
Kogut, Michael H.
Ricke, Steven C.
The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title_full The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title_fullStr The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title_full_unstemmed The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title_short The Preliminary Development of an in vitro Poultry Cecal Culture Model to Evaluate the Effects of Original XPC(TM) for the Reduction of Campylobacter jejuni and Its Potential Effects on the Microbiota
title_sort preliminary development of an in vitro poultry cecal culture model to evaluate the effects of original xpc(tm) for the reduction of campylobacter jejuni and its potential effects on the microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990144/
https://www.ncbi.nlm.nih.gov/pubmed/32038534
http://dx.doi.org/10.3389/fmicb.2019.03062
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