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SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions
[Image: see text] Zinkicide is a systemic bactericidal formulation containing protein-size fluorescent zinc oxide-based nanoparticles (nano-ZnO). Previous studies have shown that Zinkicide is effective in controlling citrus diseases. Its field performance as an antimicrobial agent has been linked to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990419/ https://www.ncbi.nlm.nih.gov/pubmed/32010811 http://dx.doi.org/10.1021/acsomega.9b02893 |
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author | Untracht, Zachary T. Ozcan, Ali Santra, Swadeshmukul Kang, Ellen H. |
author_facet | Untracht, Zachary T. Ozcan, Ali Santra, Swadeshmukul Kang, Ellen H. |
author_sort | Untracht, Zachary T. |
collection | PubMed |
description | [Image: see text] Zinkicide is a systemic bactericidal formulation containing protein-size fluorescent zinc oxide-based nanoparticles (nano-ZnO). Previous studies have shown that Zinkicide is effective in controlling citrus diseases. Its field performance as an antimicrobial agent has been linked to the bioavailability of zinc ions (Zn(2+)) at the target site. It is therefore important to monitor Zn(2+) release from Zinkicide so that application rates and frequency can be estimated. In this study, we present a simplistic approach designed to monitor Zinkicide nanoparticle dissolution rates in water and acidic buffer solutions using traditional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The evolution of nano-ZnO in the polyacrylamide gel scaffolds was studied by exciting the sample with UV light and detecting the fluorescence of nano-ZnO. Fluorescence intensities measured with this assay allowed for quantitative analysis of molecular weight changes of nano-ZnO in citrate buffer, a surrogate of citrus juice. Our results demonstrated that citrate buffer induced the greatest degradation of Zinkicide. Fluorescence intensity fluctuations were observed over time, indicating interactions of citrate with the surface of nano-ZnO. These findings provide a new approach to quantify the dissolution of nanoparticles in simulated environments, even when other analytical methods lack sensitivity because of the small size of the system (≈4 nm). |
format | Online Article Text |
id | pubmed-6990419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-69904192020-01-31 SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions Untracht, Zachary T. Ozcan, Ali Santra, Swadeshmukul Kang, Ellen H. ACS Omega [Image: see text] Zinkicide is a systemic bactericidal formulation containing protein-size fluorescent zinc oxide-based nanoparticles (nano-ZnO). Previous studies have shown that Zinkicide is effective in controlling citrus diseases. Its field performance as an antimicrobial agent has been linked to the bioavailability of zinc ions (Zn(2+)) at the target site. It is therefore important to monitor Zn(2+) release from Zinkicide so that application rates and frequency can be estimated. In this study, we present a simplistic approach designed to monitor Zinkicide nanoparticle dissolution rates in water and acidic buffer solutions using traditional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The evolution of nano-ZnO in the polyacrylamide gel scaffolds was studied by exciting the sample with UV light and detecting the fluorescence of nano-ZnO. Fluorescence intensities measured with this assay allowed for quantitative analysis of molecular weight changes of nano-ZnO in citrate buffer, a surrogate of citrus juice. Our results demonstrated that citrate buffer induced the greatest degradation of Zinkicide. Fluorescence intensity fluctuations were observed over time, indicating interactions of citrate with the surface of nano-ZnO. These findings provide a new approach to quantify the dissolution of nanoparticles in simulated environments, even when other analytical methods lack sensitivity because of the small size of the system (≈4 nm). American Chemical Society 2020-01-10 /pmc/articles/PMC6990419/ /pubmed/32010811 http://dx.doi.org/10.1021/acsomega.9b02893 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Untracht, Zachary T. Ozcan, Ali Santra, Swadeshmukul Kang, Ellen H. SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title | SDS-PAGE for Monitoring the Dissolution of Zinc Oxide
Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title_full | SDS-PAGE for Monitoring the Dissolution of Zinc Oxide
Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title_fullStr | SDS-PAGE for Monitoring the Dissolution of Zinc Oxide
Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title_full_unstemmed | SDS-PAGE for Monitoring the Dissolution of Zinc Oxide
Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title_short | SDS-PAGE for Monitoring the Dissolution of Zinc Oxide
Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions |
title_sort | sds-page for monitoring the dissolution of zinc oxide
bactericidal nanoparticles (zinkicide) in aqueous solutions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990419/ https://www.ncbi.nlm.nih.gov/pubmed/32010811 http://dx.doi.org/10.1021/acsomega.9b02893 |
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