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Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast

[Image: see text] Abused or misused carbadox (CBX) and cyadox (CYA) in animal feed may cause food safety concerns, threatening human health. Here, we describe the design of a novel hapten for preparation of a monoclonal antibody against CBX and CYA simultaneously. Using this antibody, colloidal gold...

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Autores principales: Guo, Lingling, Wu, Xiaoling, Cui, Gang, Song, Shanshan, Kuang, Hua, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990421/
https://www.ncbi.nlm.nih.gov/pubmed/32010814
http://dx.doi.org/10.1021/acsomega.9b02931
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author Guo, Lingling
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
author_facet Guo, Lingling
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
author_sort Guo, Lingling
collection PubMed
description [Image: see text] Abused or misused carbadox (CBX) and cyadox (CYA) in animal feed may cause food safety concerns, threatening human health. Here, we describe the design of a novel hapten for preparation of a monoclonal antibody against CBX and CYA simultaneously. Using this antibody, colloidal gold immunochromatographic assay (GICA) was developed for screening of CBX and CYA residues in chicken breast. Under optimal conditions, semiquantitative analysis results were visible by eye, with a visual limit of detection of 8 μg/kg for CBX and CYA, and cut-off values of 20 μg/kg for CBX and 40 μg/kg for CYA in chicken breast. Quantitative analysis could be performed using a hand-held strip scanner, with a calculated limit of detection of 2.92 μg/kg for CBX and 2.68 μg/kg for CYA in chicken breast. Validated by liquid chromatography–MS/MS, the developed GICA provides a useful tool for rapid on-site CBX and CYA residue screening in chicken breast.
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spelling pubmed-69904212020-01-31 Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast Guo, Lingling Wu, Xiaoling Cui, Gang Song, Shanshan Kuang, Hua Xu, Chuanlai ACS Omega [Image: see text] Abused or misused carbadox (CBX) and cyadox (CYA) in animal feed may cause food safety concerns, threatening human health. Here, we describe the design of a novel hapten for preparation of a monoclonal antibody against CBX and CYA simultaneously. Using this antibody, colloidal gold immunochromatographic assay (GICA) was developed for screening of CBX and CYA residues in chicken breast. Under optimal conditions, semiquantitative analysis results were visible by eye, with a visual limit of detection of 8 μg/kg for CBX and CYA, and cut-off values of 20 μg/kg for CBX and 40 μg/kg for CYA in chicken breast. Quantitative analysis could be performed using a hand-held strip scanner, with a calculated limit of detection of 2.92 μg/kg for CBX and 2.68 μg/kg for CYA in chicken breast. Validated by liquid chromatography–MS/MS, the developed GICA provides a useful tool for rapid on-site CBX and CYA residue screening in chicken breast. American Chemical Society 2020-01-13 /pmc/articles/PMC6990421/ /pubmed/32010814 http://dx.doi.org/10.1021/acsomega.9b02931 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Guo, Lingling
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title_full Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title_fullStr Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title_full_unstemmed Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title_short Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast
title_sort colloidal gold immunochromatographic assay for rapid detection of carbadox and cyadox in chicken breast
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990421/
https://www.ncbi.nlm.nih.gov/pubmed/32010814
http://dx.doi.org/10.1021/acsomega.9b02931
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