Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

[Image: see text] In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC(50)) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (I...

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Autores principales: Lin, Lu, Wu, Xiaoling, Cui, Gang, Song, Shanshan, Kuang, Hua, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990434/
https://www.ncbi.nlm.nih.gov/pubmed/32010804
http://dx.doi.org/10.1021/acsomega.9b01841
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author Lin, Lu
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
author_facet Lin, Lu
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
author_sort Lin, Lu
collection PubMed
description [Image: see text] In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC(50)) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (IC(20)–IC(80)) was 0.165–1.29 ng/mL. Based on this anti-azaperone mAb, a colloidal gold immunochromatographic strip assay method was established for the detection of azaperone in pork and pork liver samples. For pork samples, the cutoff value of the colloidal gold immunochromatographic strip assay was 10 ng/g, and the cutoff value was also 10 ng/g in pig liver samples. This colloidal gold immunochromatographic strip assay is a rapid, simple, and practical method for the detection of azaperone in pork and pork liver samples available in markets.
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spelling pubmed-69904342020-01-31 Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver Lin, Lu Wu, Xiaoling Cui, Gang Song, Shanshan Kuang, Hua Xu, Chuanlai ACS Omega [Image: see text] In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC(50)) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (IC(20)–IC(80)) was 0.165–1.29 ng/mL. Based on this anti-azaperone mAb, a colloidal gold immunochromatographic strip assay method was established for the detection of azaperone in pork and pork liver samples. For pork samples, the cutoff value of the colloidal gold immunochromatographic strip assay was 10 ng/g, and the cutoff value was also 10 ng/g in pig liver samples. This colloidal gold immunochromatographic strip assay is a rapid, simple, and practical method for the detection of azaperone in pork and pork liver samples available in markets. American Chemical Society 2020-01-13 /pmc/articles/PMC6990434/ /pubmed/32010804 http://dx.doi.org/10.1021/acsomega.9b01841 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Lin, Lu
Wu, Xiaoling
Cui, Gang
Song, Shanshan
Kuang, Hua
Xu, Chuanlai
Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title_full Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title_fullStr Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title_full_unstemmed Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title_short Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver
title_sort colloidal gold immunochromatographic strip assay for the detection of azaperone in pork and pork liver
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990434/
https://www.ncbi.nlm.nih.gov/pubmed/32010804
http://dx.doi.org/10.1021/acsomega.9b01841
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