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Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days

BACKGROUND: Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytomet...

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Autores principales: Lasta, Camila Serina, Hlavac, Nicole, Marcondes, Natália Aydos, Dalmolin, Magnus Larruscaim, Terra, Silvia Resende, de Almeida Lacerda, Luciana, Faulhaber, Gustavo Adolpho Moreira, González, Félix Hilário Díaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990466/
https://www.ncbi.nlm.nih.gov/pubmed/32000762
http://dx.doi.org/10.1186/s12917-020-2254-5
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author Lasta, Camila Serina
Hlavac, Nicole
Marcondes, Natália Aydos
Dalmolin, Magnus Larruscaim
Terra, Silvia Resende
de Almeida Lacerda, Luciana
Faulhaber, Gustavo Adolpho Moreira
González, Félix Hilário Díaz
author_facet Lasta, Camila Serina
Hlavac, Nicole
Marcondes, Natália Aydos
Dalmolin, Magnus Larruscaim
Terra, Silvia Resende
de Almeida Lacerda, Luciana
Faulhaber, Gustavo Adolpho Moreira
González, Félix Hilário Díaz
author_sort Lasta, Camila Serina
collection PubMed
description BACKGROUND: Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytometry and biochemistry assays. Twenty-two bags of canine platelet concentrates were obtained by the platelet-rich plasma method and were assessed on days 1, 3 and 5 after collection. Parameters such as platelet counts, residual leukocytes, platelet swirling, glucose, lactate, pH, CD62P expression (platelet activation), JC-1 (mitochondrial function) and annexin V (apoptosis and cell death) were assessed. RESULTS: Over the five days of storage there was a significant decrease in glucose, HCO(3), pCO(2), ATP, pH, swirling and mitochondrial function, associated with a significant increase in lactate levels and pO(2). At the end of storage pH was 5.9 ± 0.6 and lactate levels were 2.8 ± 1.2 mmol/L. Results of the quality parameters evaluated were similar to those reported in human platelets studies. The deleterious effects of storage were more pronounced in bags with higher platelet counts (> 7.49 × 10(10)/unit), suggesting that canine platelet concentrates should not contain an excessive number of platelets. CONCLUSIONS: Quality parameters of canine platelets under standard storage conditions were similar to those observed in human platelets. Our results have potential to be used for the routine evaluation and quality control in veterinary blood banks.
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spelling pubmed-69904662020-02-03 Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days Lasta, Camila Serina Hlavac, Nicole Marcondes, Natália Aydos Dalmolin, Magnus Larruscaim Terra, Silvia Resende de Almeida Lacerda, Luciana Faulhaber, Gustavo Adolpho Moreira González, Félix Hilário Díaz BMC Vet Res Research Article BACKGROUND: Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytometry and biochemistry assays. Twenty-two bags of canine platelet concentrates were obtained by the platelet-rich plasma method and were assessed on days 1, 3 and 5 after collection. Parameters such as platelet counts, residual leukocytes, platelet swirling, glucose, lactate, pH, CD62P expression (platelet activation), JC-1 (mitochondrial function) and annexin V (apoptosis and cell death) were assessed. RESULTS: Over the five days of storage there was a significant decrease in glucose, HCO(3), pCO(2), ATP, pH, swirling and mitochondrial function, associated with a significant increase in lactate levels and pO(2). At the end of storage pH was 5.9 ± 0.6 and lactate levels were 2.8 ± 1.2 mmol/L. Results of the quality parameters evaluated were similar to those reported in human platelets studies. The deleterious effects of storage were more pronounced in bags with higher platelet counts (> 7.49 × 10(10)/unit), suggesting that canine platelet concentrates should not contain an excessive number of platelets. CONCLUSIONS: Quality parameters of canine platelets under standard storage conditions were similar to those observed in human platelets. Our results have potential to be used for the routine evaluation and quality control in veterinary blood banks. BioMed Central 2020-01-30 /pmc/articles/PMC6990466/ /pubmed/32000762 http://dx.doi.org/10.1186/s12917-020-2254-5 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lasta, Camila Serina
Hlavac, Nicole
Marcondes, Natália Aydos
Dalmolin, Magnus Larruscaim
Terra, Silvia Resende
de Almeida Lacerda, Luciana
Faulhaber, Gustavo Adolpho Moreira
González, Félix Hilário Díaz
Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title_full Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title_fullStr Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title_full_unstemmed Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title_short Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
title_sort quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990466/
https://www.ncbi.nlm.nih.gov/pubmed/32000762
http://dx.doi.org/10.1186/s12917-020-2254-5
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