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Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE
Intracellular vesicle fusion is mediated by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18 (SM) proteins. It is generally accepted that membrane fusion occurs when the vesicle and target membranes are brought into close proximity by SNAREs and SM prot...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990648/ https://www.ncbi.nlm.nih.gov/pubmed/31875562 http://dx.doi.org/10.1016/j.celrep.2019.11.107 |
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author | Rathore, Shailendra S. Liu, Yinghui Yu, Haijia Wan, Chun Lee, MyeongSeon Yin, Qian Stowell, Michael H.B. Shen, Jingshi |
author_facet | Rathore, Shailendra S. Liu, Yinghui Yu, Haijia Wan, Chun Lee, MyeongSeon Yin, Qian Stowell, Michael H.B. Shen, Jingshi |
author_sort | Rathore, Shailendra S. |
collection | PubMed |
description | Intracellular vesicle fusion is mediated by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18 (SM) proteins. It is generally accepted that membrane fusion occurs when the vesicle and target membranes are brought into close proximity by SNAREs and SM proteins. In this work, we demonstrate that, for fusion to occur, membrane bilayers must be destabilized by a conserved membrane-embedded motif located at the juxtamembrane region of the vesicle-anchored v-SNARE. Comprised of basic and hydrophobic residues, the juxtamembrane motif perturbs the lipid bilayer structure and promotes SNARE-SM-mediated membrane fusion. The juxtamembrane motif can be functionally substituted with an unrelated membrane-disrupting peptide in the membrane fusion reaction. These findings establish the juxtamembrane motif of the v-SNARE as a membrane-destabilizing peptide. Requirement of membrane-destabilizing peptides is likely a common feature of biological membrane fusion. |
format | Online Article Text |
id | pubmed-6990648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-69906482020-01-30 Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE Rathore, Shailendra S. Liu, Yinghui Yu, Haijia Wan, Chun Lee, MyeongSeon Yin, Qian Stowell, Michael H.B. Shen, Jingshi Cell Rep Article Intracellular vesicle fusion is mediated by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18 (SM) proteins. It is generally accepted that membrane fusion occurs when the vesicle and target membranes are brought into close proximity by SNAREs and SM proteins. In this work, we demonstrate that, for fusion to occur, membrane bilayers must be destabilized by a conserved membrane-embedded motif located at the juxtamembrane region of the vesicle-anchored v-SNARE. Comprised of basic and hydrophobic residues, the juxtamembrane motif perturbs the lipid bilayer structure and promotes SNARE-SM-mediated membrane fusion. The juxtamembrane motif can be functionally substituted with an unrelated membrane-disrupting peptide in the membrane fusion reaction. These findings establish the juxtamembrane motif of the v-SNARE as a membrane-destabilizing peptide. Requirement of membrane-destabilizing peptides is likely a common feature of biological membrane fusion. 2019-12-24 /pmc/articles/PMC6990648/ /pubmed/31875562 http://dx.doi.org/10.1016/j.celrep.2019.11.107 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Rathore, Shailendra S. Liu, Yinghui Yu, Haijia Wan, Chun Lee, MyeongSeon Yin, Qian Stowell, Michael H.B. Shen, Jingshi Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title | Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title_full | Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title_fullStr | Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title_full_unstemmed | Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title_short | Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE |
title_sort | intracellular vesicle fusion requires a membrane-destabilizing peptide located at the juxtamembrane region of the v-snare |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990648/ https://www.ncbi.nlm.nih.gov/pubmed/31875562 http://dx.doi.org/10.1016/j.celrep.2019.11.107 |
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