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The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China

Banana streak virus (BSV) belongs to the members of the genus Badnavirus, family Caulimoviridae. At present, BSV contains nine species in the International Committee on Taxonomy of Viruses (ICTV) classification report (2018b release). Previous study indicated that the viral particles of Banana strea...

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Autores principales: Li, Wei-li, Yu, Nai-tong, Wang, Jian-hua, Li, Jun-cheng, Liu, Zhi-xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991131/
https://www.ncbi.nlm.nih.gov/pubmed/32025380
http://dx.doi.org/10.7717/peerj.8459
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author Li, Wei-li
Yu, Nai-tong
Wang, Jian-hua
Li, Jun-cheng
Liu, Zhi-xin
author_facet Li, Wei-li
Yu, Nai-tong
Wang, Jian-hua
Li, Jun-cheng
Liu, Zhi-xin
author_sort Li, Wei-li
collection PubMed
description Banana streak virus (BSV) belongs to the members of the genus Badnavirus, family Caulimoviridae. At present, BSV contains nine species in the International Committee on Taxonomy of Viruses (ICTV) classification report (2018b release). Previous study indicated that the viral particles of Banana streak virus Acuminata Yunnan (BSV-Acum) were purified from banana (Cavendish Musa AAA group) leaves in Yunnan Province, China, and its complete genome was obtained. To further determine whether this sample infecting with Banana streak GF virus (BSGFV), the polymerase chain reaction (PCR) cloning and complete genome analysis of the Banana streak GF virus Yunnan isolate (BSGFV-YN) isolate were carried out in this study. The result showed that BSGFV-YN infecting Cavendish Musa AAA group was co-infecting this sample. Its genome contains a total of 7,325 bp in length with 42% GC content. This complete genome sequence was deposited in GenBank under accession number MN296502. Sequence analysis showed that the complete genome of BSGFV-YN was 98.14% sequence similarity to BSGFV Goldfinger, while it was 49.10–57.09% to other BSV species. Two phylogenetic trees based on the complete genome and ORFIII polyprotein indicated that BSGFV-YN and other BSV species clustered into a group, while it was the highest homology with BSGFV Goldfinger. Although BSGFV-YN and BSGFV Goldfinger were highly homologous, their cultivating bananas are different. The former cultivating banana was from Cavendish Musa AAA group, while the latter cultivating banana was from Goldfinger Musa AAAB group. Compared with BSGFV Goldfinger, the genome of BSGFV-YN has an extra multiple repetitive sequences in the intergenetic region between ORFIII and ORFI, suggesting that this region might be related to host selection. In summary, a BSGFV-YN distant from BSV-Acum was identified from the same sample, and its complete genome sequence was determined and analyzed. The study extends the polymorphism of BSVs in China and provides scientific clue for the evolutionary relationship with host selection of badnaviruses.
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spelling pubmed-69911312020-02-05 The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China Li, Wei-li Yu, Nai-tong Wang, Jian-hua Li, Jun-cheng Liu, Zhi-xin PeerJ Genomics Banana streak virus (BSV) belongs to the members of the genus Badnavirus, family Caulimoviridae. At present, BSV contains nine species in the International Committee on Taxonomy of Viruses (ICTV) classification report (2018b release). Previous study indicated that the viral particles of Banana streak virus Acuminata Yunnan (BSV-Acum) were purified from banana (Cavendish Musa AAA group) leaves in Yunnan Province, China, and its complete genome was obtained. To further determine whether this sample infecting with Banana streak GF virus (BSGFV), the polymerase chain reaction (PCR) cloning and complete genome analysis of the Banana streak GF virus Yunnan isolate (BSGFV-YN) isolate were carried out in this study. The result showed that BSGFV-YN infecting Cavendish Musa AAA group was co-infecting this sample. Its genome contains a total of 7,325 bp in length with 42% GC content. This complete genome sequence was deposited in GenBank under accession number MN296502. Sequence analysis showed that the complete genome of BSGFV-YN was 98.14% sequence similarity to BSGFV Goldfinger, while it was 49.10–57.09% to other BSV species. Two phylogenetic trees based on the complete genome and ORFIII polyprotein indicated that BSGFV-YN and other BSV species clustered into a group, while it was the highest homology with BSGFV Goldfinger. Although BSGFV-YN and BSGFV Goldfinger were highly homologous, their cultivating bananas are different. The former cultivating banana was from Cavendish Musa AAA group, while the latter cultivating banana was from Goldfinger Musa AAAB group. Compared with BSGFV Goldfinger, the genome of BSGFV-YN has an extra multiple repetitive sequences in the intergenetic region between ORFIII and ORFI, suggesting that this region might be related to host selection. In summary, a BSGFV-YN distant from BSV-Acum was identified from the same sample, and its complete genome sequence was determined and analyzed. The study extends the polymorphism of BSVs in China and provides scientific clue for the evolutionary relationship with host selection of badnaviruses. PeerJ Inc. 2020-01-27 /pmc/articles/PMC6991131/ /pubmed/32025380 http://dx.doi.org/10.7717/peerj.8459 Text en ©2020 Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genomics
Li, Wei-li
Yu, Nai-tong
Wang, Jian-hua
Li, Jun-cheng
Liu, Zhi-xin
The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title_full The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title_fullStr The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title_full_unstemmed The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title_short The complete genome of Banana streak GF virus Yunnan isolate infecting Cavendish Musa AAA group in China
title_sort complete genome of banana streak gf virus yunnan isolate infecting cavendish musa aaa group in china
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991131/
https://www.ncbi.nlm.nih.gov/pubmed/32025380
http://dx.doi.org/10.7717/peerj.8459
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