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Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus

Although fluorescence tracing of small bioactive molecules in living cells has been extensively studied, it is still a challenging task to detect their variations in the nucleus mainly due to the impermeable nuclear membrane and nucleic acid interference. Herein, we take advantage of the nucleic aci...

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Autores principales: Wang, Kang-Nan, Cao, Qian, Liu, Liu-Yi, Zhao, Zi-Jian, Liu, Wenting, Zhou, Dan-Jie, Tan, Cai-Ping, Xia, Wei, Ji, Liang-Nian, Mao, Zong-Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991190/
https://www.ncbi.nlm.nih.gov/pubmed/32055359
http://dx.doi.org/10.1039/c9sc03594j
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author Wang, Kang-Nan
Cao, Qian
Liu, Liu-Yi
Zhao, Zi-Jian
Liu, Wenting
Zhou, Dan-Jie
Tan, Cai-Ping
Xia, Wei
Ji, Liang-Nian
Mao, Zong-Wan
author_facet Wang, Kang-Nan
Cao, Qian
Liu, Liu-Yi
Zhao, Zi-Jian
Liu, Wenting
Zhou, Dan-Jie
Tan, Cai-Ping
Xia, Wei
Ji, Liang-Nian
Mao, Zong-Wan
author_sort Wang, Kang-Nan
collection PubMed
description Although fluorescence tracing of small bioactive molecules in living cells has been extensively studied, it is still a challenging task to detect their variations in the nucleus mainly due to the impermeable nuclear membrane and nucleic acid interference. Herein, we take advantage of the nucleic acid enriched environment in the nucleus to establish a strategy, named “charge-driven tripod somersault on DNA”, for ratiometric fluorescence imaging of small bioactive molecules in the nucleus. Taking SO(2) derivatives as a typical target analyte, a tripodal probe has been constructed by conjugating two DNA binding groups containing a SO(2) derivative reaction site. Mechanism studies demonstrate that upon encountering and reacting with SO(3)(2–)/HSO(3)(–), a charge variation occurs at the responsive arm of the tripodal probe, triggering a tripod somersault on DNA, resulting in the conformational rearrangement of the DNA binding modes with DNA-modulated fluorescence change, which allows the second emission feature to emerge. In this strategy, probe–DNA binding is not influenced by RNA or non-specific protein association, thus making it ideal for tracing nucleus-localized analytes. The application of this strategy has realized both in vitro and in vivo ratiometric fluorescence imaging of the variations of endogenous SO(2) derivatives in the nucleus for the first time, with high specificity and selectivity. Also, in theory, this strategy opens up a new avenue for the design of fluorescence probes for the nucleus-localized biological analytes.
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spelling pubmed-69911902020-02-13 Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus Wang, Kang-Nan Cao, Qian Liu, Liu-Yi Zhao, Zi-Jian Liu, Wenting Zhou, Dan-Jie Tan, Cai-Ping Xia, Wei Ji, Liang-Nian Mao, Zong-Wan Chem Sci Chemistry Although fluorescence tracing of small bioactive molecules in living cells has been extensively studied, it is still a challenging task to detect their variations in the nucleus mainly due to the impermeable nuclear membrane and nucleic acid interference. Herein, we take advantage of the nucleic acid enriched environment in the nucleus to establish a strategy, named “charge-driven tripod somersault on DNA”, for ratiometric fluorescence imaging of small bioactive molecules in the nucleus. Taking SO(2) derivatives as a typical target analyte, a tripodal probe has been constructed by conjugating two DNA binding groups containing a SO(2) derivative reaction site. Mechanism studies demonstrate that upon encountering and reacting with SO(3)(2–)/HSO(3)(–), a charge variation occurs at the responsive arm of the tripodal probe, triggering a tripod somersault on DNA, resulting in the conformational rearrangement of the DNA binding modes with DNA-modulated fluorescence change, which allows the second emission feature to emerge. In this strategy, probe–DNA binding is not influenced by RNA or non-specific protein association, thus making it ideal for tracing nucleus-localized analytes. The application of this strategy has realized both in vitro and in vivo ratiometric fluorescence imaging of the variations of endogenous SO(2) derivatives in the nucleus for the first time, with high specificity and selectivity. Also, in theory, this strategy opens up a new avenue for the design of fluorescence probes for the nucleus-localized biological analytes. Royal Society of Chemistry 2019-09-06 /pmc/articles/PMC6991190/ /pubmed/32055359 http://dx.doi.org/10.1039/c9sc03594j Text en This journal is © The Royal Society of Chemistry 2019 https://creativecommons.org/licenses/by-nc/3.0/This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Wang, Kang-Nan
Cao, Qian
Liu, Liu-Yi
Zhao, Zi-Jian
Liu, Wenting
Zhou, Dan-Jie
Tan, Cai-Ping
Xia, Wei
Ji, Liang-Nian
Mao, Zong-Wan
Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title_full Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title_fullStr Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title_full_unstemmed Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title_short Charge-driven tripod somersault on DNA for ratiometric fluorescence imaging of small molecules in the nucleus
title_sort charge-driven tripod somersault on dna for ratiometric fluorescence imaging of small molecules in the nucleus
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991190/
https://www.ncbi.nlm.nih.gov/pubmed/32055359
http://dx.doi.org/10.1039/c9sc03594j
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