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The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion

The skin expansion technique is widely used to induce skin growth for large‐scale skin deformity reconstruction. However, the capacity for skin expansion is limited and searching for ways to improve the expansion efficiency is a challenge. In this study, we aimed to explore the possible mechanism of...

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Autores principales: Zhou, Yiwen, Li, Haizhou, Liang, Xiao, Du, Hengyu, Suo, Yingjun, Chen, Hao, Liu, Wenhui, Duan, Ran, Huang, Xiaolu, Li, Qingfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991652/
https://www.ncbi.nlm.nih.gov/pubmed/31828970
http://dx.doi.org/10.1111/jcmm.14828
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author Zhou, Yiwen
Li, Haizhou
Liang, Xiao
Du, Hengyu
Suo, Yingjun
Chen, Hao
Liu, Wenhui
Duan, Ran
Huang, Xiaolu
Li, Qingfeng
author_facet Zhou, Yiwen
Li, Haizhou
Liang, Xiao
Du, Hengyu
Suo, Yingjun
Chen, Hao
Liu, Wenhui
Duan, Ran
Huang, Xiaolu
Li, Qingfeng
author_sort Zhou, Yiwen
collection PubMed
description The skin expansion technique is widely used to induce skin growth for large‐scale skin deformity reconstruction. However, the capacity for skin expansion is limited and searching for ways to improve the expansion efficiency is a challenge. In this study, we aimed to explore the possible mechanism of skin expansion and to find a potential therapeutic target on promoting skin growth. We conducted weighted gene coexpression network analysis (WGCNA) of microarray data generated from rat skin expansion and found CCN1 (CYR61) to be the central hub gene related to epithelial‐mesenchymal transition (EMT). CCN1 up‐regulation was confirmed in human and rat expanded skin and also in mechanically stretched rat keratinocytes, together with acquired mesenchymal phenotype. After CCN1 stimulation on keratinocytes, cell proliferation was promoted and partial EMT was induced by activating β‐catenin pathway. Treatment of CCN1 protein could significantly increase the flap thickness, improve the blood supply and restore the structure in a rat model of skin expansion, whereas inhibition of CCN1 through shRNA interference could dramatically reduce the efficiency of skin expansion. Our findings demonstrate that CCN1 plays a crucial role in skin expansion and that CCN1 may serve as a potential therapeutic target to promote skin growth and improve the efficiency of skin expansion.
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spelling pubmed-69916522020-02-03 The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion Zhou, Yiwen Li, Haizhou Liang, Xiao Du, Hengyu Suo, Yingjun Chen, Hao Liu, Wenhui Duan, Ran Huang, Xiaolu Li, Qingfeng J Cell Mol Med Original Articles The skin expansion technique is widely used to induce skin growth for large‐scale skin deformity reconstruction. However, the capacity for skin expansion is limited and searching for ways to improve the expansion efficiency is a challenge. In this study, we aimed to explore the possible mechanism of skin expansion and to find a potential therapeutic target on promoting skin growth. We conducted weighted gene coexpression network analysis (WGCNA) of microarray data generated from rat skin expansion and found CCN1 (CYR61) to be the central hub gene related to epithelial‐mesenchymal transition (EMT). CCN1 up‐regulation was confirmed in human and rat expanded skin and also in mechanically stretched rat keratinocytes, together with acquired mesenchymal phenotype. After CCN1 stimulation on keratinocytes, cell proliferation was promoted and partial EMT was induced by activating β‐catenin pathway. Treatment of CCN1 protein could significantly increase the flap thickness, improve the blood supply and restore the structure in a rat model of skin expansion, whereas inhibition of CCN1 through shRNA interference could dramatically reduce the efficiency of skin expansion. Our findings demonstrate that CCN1 plays a crucial role in skin expansion and that CCN1 may serve as a potential therapeutic target to promote skin growth and improve the efficiency of skin expansion. John Wiley and Sons Inc. 2019-12-11 2020-01 /pmc/articles/PMC6991652/ /pubmed/31828970 http://dx.doi.org/10.1111/jcmm.14828 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhou, Yiwen
Li, Haizhou
Liang, Xiao
Du, Hengyu
Suo, Yingjun
Chen, Hao
Liu, Wenhui
Duan, Ran
Huang, Xiaolu
Li, Qingfeng
The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title_full The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title_fullStr The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title_full_unstemmed The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title_short The CCN1 (CYR61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
title_sort ccn1 (cyr61) protein promotes skin growth by enhancing epithelial‐mesenchymal transition during skin expansion
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991652/
https://www.ncbi.nlm.nih.gov/pubmed/31828970
http://dx.doi.org/10.1111/jcmm.14828
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