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Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades
Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods tha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992001/ https://www.ncbi.nlm.nih.gov/pubmed/31999773 http://dx.doi.org/10.1371/journal.pone.0228325 |
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author | Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián |
author_facet | Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián |
author_sort | Chiumiento, Ignacio Rafael |
collection | PubMed |
description | Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin. |
format | Online Article Text |
id | pubmed-6992001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69920012020-02-20 Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián PLoS One Research Article Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin. Public Library of Science 2020-01-30 /pmc/articles/PMC6992001/ /pubmed/31999773 http://dx.doi.org/10.1371/journal.pone.0228325 Text en © 2020 Chiumiento et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chiumiento, Ignacio Rafael Ituarte, Santiago Sun, Jin Qiu, Jian Wen Heras, Horacio Dreon, Marcos Sebastián Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title | Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title_full | Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title_fullStr | Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title_full_unstemmed | Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title_short | Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades |
title_sort | hemocyanin of the caenogastropod pomacea canaliculata exhibits evolutionary differences among gastropod clades |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992001/ https://www.ncbi.nlm.nih.gov/pubmed/31999773 http://dx.doi.org/10.1371/journal.pone.0228325 |
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