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Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

DNA from formalin-preserved tissue could unlock a vast repository of genetic information stored in museums worldwide. However, formaldehyde crosslinks proteins and DNA, and prevents ready amplification and DNA sequencing. Formaldehyde acylation also fragments the DNA. Treatment with proteinase K pro...

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Detalles Bibliográficos
Autores principales:	Totoiu, Christian A., Phillips, Jessica M., Reese, Aspen T., Majumdar, Sudipta, Girguis, Peter R., Raston, Colin L., Weiss, Gregory A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992170/ https://www.ncbi.nlm.nih.gov/pubmed/31999723 http://dx.doi.org/10.1371/journal.pone.0225807

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