Imaging brain activity during complex social behaviors in Drosophila with Flyception2

Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male...

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Autores principales: Grover, Dhruv, Katsuki, Takeo, Li, Jinfang, Dawkins, Thomas J., Greenspan, Ralph J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992788/
https://www.ncbi.nlm.nih.gov/pubmed/32001689
http://dx.doi.org/10.1038/s41467-020-14487-7
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author Grover, Dhruv
Katsuki, Takeo
Li, Jinfang
Dawkins, Thomas J.
Greenspan, Ralph J.
author_facet Grover, Dhruv
Katsuki, Takeo
Li, Jinfang
Dawkins, Thomas J.
Greenspan, Ralph J.
author_sort Grover, Dhruv
collection PubMed
description Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating.
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spelling pubmed-69927882020-02-03 Imaging brain activity during complex social behaviors in Drosophila with Flyception2 Grover, Dhruv Katsuki, Takeo Li, Jinfang Dawkins, Thomas J. Greenspan, Ralph J. Nat Commun Article Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating. Nature Publishing Group UK 2020-01-30 /pmc/articles/PMC6992788/ /pubmed/32001689 http://dx.doi.org/10.1038/s41467-020-14487-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Grover, Dhruv
Katsuki, Takeo
Li, Jinfang
Dawkins, Thomas J.
Greenspan, Ralph J.
Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title_full Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title_fullStr Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title_full_unstemmed Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title_short Imaging brain activity during complex social behaviors in Drosophila with Flyception2
title_sort imaging brain activity during complex social behaviors in drosophila with flyception2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992788/
https://www.ncbi.nlm.nih.gov/pubmed/32001689
http://dx.doi.org/10.1038/s41467-020-14487-7
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