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Optimization of overlap extension PCR for efficient transgene construction

PCR is a powerful tool for generating specific fragments of DNA that can be used to create gene variations or tagged expression constructs. Overlap extension PCR is a valuable technique that is commonly used for cloning large complex fragments, making edits to cloned genes or fusing two gene element...

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Detalles Bibliográficos
Autores principales:	Hilgarth, Roland S., Lanigan, Thomas M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2019
Materias:	Biochemistry, Genetics and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992990/ https://www.ncbi.nlm.nih.gov/pubmed/32021819 http://dx.doi.org/10.1016/j.mex.2019.12.001

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