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Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth

Ribulose-1,5-bisphosphate carboxylase/oxygenase, the key enzyme of photosynthetic carbon fixation, is able to accept both O(2) and CO(2) as substrates. When it fixes O(2), it produces 2-phosphoglycolate, which is detoxified by photorespiration and recycled to the Calvin–Benson–Bassham cycle. To comp...

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Autores principales: Shim, Su-Hyeon, Lee, Sang-Kyu, Lee, Dae-Woo, Brilhaus, Dominik, Wu, Guangxi, Ko, Sooyeon, Lee, Choon-Hwan, Weber, Andreas P.M., Jeon, Jong-Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993116/
https://www.ncbi.nlm.nih.gov/pubmed/32038690
http://dx.doi.org/10.3389/fpls.2019.01726
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author Shim, Su-Hyeon
Lee, Sang-Kyu
Lee, Dae-Woo
Brilhaus, Dominik
Wu, Guangxi
Ko, Sooyeon
Lee, Choon-Hwan
Weber, Andreas P.M.
Jeon, Jong-Seong
author_facet Shim, Su-Hyeon
Lee, Sang-Kyu
Lee, Dae-Woo
Brilhaus, Dominik
Wu, Guangxi
Ko, Sooyeon
Lee, Choon-Hwan
Weber, Andreas P.M.
Jeon, Jong-Seong
author_sort Shim, Su-Hyeon
collection PubMed
description Ribulose-1,5-bisphosphate carboxylase/oxygenase, the key enzyme of photosynthetic carbon fixation, is able to accept both O(2) and CO(2) as substrates. When it fixes O(2), it produces 2-phosphoglycolate, which is detoxified by photorespiration and recycled to the Calvin–Benson–Bassham cycle. To complete photorespiration, metabolite transport across three organelles, chloroplasts, peroxisomes, and mitochondria, is necessary through transmembrane transporters. In rice (Oryza sativa) little is known about photorespiratory transmembrane transporters. Here, we identified the rice plastidic glycolate/glycerate translocator 1 (OsPLGG1), a homolog of Arabidopsis PLGG1. OsPLGG1 mutant lines, osplgg1-1, osplgg1-2, and osplgg1-3, showed a growth retardation phenotype, such as pale green leaf, reduced tiller number, and reduced seed grain weight as well as reduced photosynthetic carbon reduction rate due to low activities of photosystem I and II. The plant growth retardation in osplgg1 mutants was rescued under high CO(2) condition. Subcellular localization of OsPLGG1–GFP fusion protein, along with its predicted N-terminal transmembrane domain, confirmed that OsPLGG1 is a chloroplast transmembrane protein. Metabolite analysis indicated significant accumulation of photorespiratory metabolites, especially glycolate and glycerate, which have been shown to be transported by the Arabidopsis PLGG1, and changes for a number of metabolites which are not intermediates of photorespiration in the mutants. These results suggest that OsPLGG1 is the functional plastidic glycolate/glycerate transporter, which is necessary for photorespiration and growth in rice.
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spelling pubmed-69931162020-02-07 Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth Shim, Su-Hyeon Lee, Sang-Kyu Lee, Dae-Woo Brilhaus, Dominik Wu, Guangxi Ko, Sooyeon Lee, Choon-Hwan Weber, Andreas P.M. Jeon, Jong-Seong Front Plant Sci Plant Science Ribulose-1,5-bisphosphate carboxylase/oxygenase, the key enzyme of photosynthetic carbon fixation, is able to accept both O(2) and CO(2) as substrates. When it fixes O(2), it produces 2-phosphoglycolate, which is detoxified by photorespiration and recycled to the Calvin–Benson–Bassham cycle. To complete photorespiration, metabolite transport across three organelles, chloroplasts, peroxisomes, and mitochondria, is necessary through transmembrane transporters. In rice (Oryza sativa) little is known about photorespiratory transmembrane transporters. Here, we identified the rice plastidic glycolate/glycerate translocator 1 (OsPLGG1), a homolog of Arabidopsis PLGG1. OsPLGG1 mutant lines, osplgg1-1, osplgg1-2, and osplgg1-3, showed a growth retardation phenotype, such as pale green leaf, reduced tiller number, and reduced seed grain weight as well as reduced photosynthetic carbon reduction rate due to low activities of photosystem I and II. The plant growth retardation in osplgg1 mutants was rescued under high CO(2) condition. Subcellular localization of OsPLGG1–GFP fusion protein, along with its predicted N-terminal transmembrane domain, confirmed that OsPLGG1 is a chloroplast transmembrane protein. Metabolite analysis indicated significant accumulation of photorespiratory metabolites, especially glycolate and glycerate, which have been shown to be transported by the Arabidopsis PLGG1, and changes for a number of metabolites which are not intermediates of photorespiration in the mutants. These results suggest that OsPLGG1 is the functional plastidic glycolate/glycerate transporter, which is necessary for photorespiration and growth in rice. Frontiers Media S.A. 2020-01-24 /pmc/articles/PMC6993116/ /pubmed/32038690 http://dx.doi.org/10.3389/fpls.2019.01726 Text en Copyright © 2020 Shim, Lee, Lee, Brilhaus, Wu, Ko, Lee, Weber and Jeon http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shim, Su-Hyeon
Lee, Sang-Kyu
Lee, Dae-Woo
Brilhaus, Dominik
Wu, Guangxi
Ko, Sooyeon
Lee, Choon-Hwan
Weber, Andreas P.M.
Jeon, Jong-Seong
Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title_full Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title_fullStr Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title_full_unstemmed Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title_short Loss of Function of Rice Plastidic Glycolate/Glycerate Translocator 1 Impairs Photorespiration and Plant Growth
title_sort loss of function of rice plastidic glycolate/glycerate translocator 1 impairs photorespiration and plant growth
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993116/
https://www.ncbi.nlm.nih.gov/pubmed/32038690
http://dx.doi.org/10.3389/fpls.2019.01726
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