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Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice
OBJECTIVE: The aim of this study was to investigate the efficacy of protocols for mice ovary cryopreservation to compare the differences in Mouse Vasa Homologue expression (a germline cell marker) and ovarian viability after vitrification or slow freezing. METHODS: Female CF1 mice aged 40-45 days we...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Assisted Reproduction
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993165/ https://www.ncbi.nlm.nih.gov/pubmed/31689043 http://dx.doi.org/10.5935/1518-0557.20190057 |
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author | Terraciano, Paula Barros Garcez, Tuane Alves Berger, Markus Durli, Isabel Kuhl, Cristiana Palma Batista, Vitória de Oliveira Schneider, Raquel de Almeida Festa, Jaquelline Pilar, Emily Goulart, Marcela Ferreira, Charles Passos, Eduardo Pandolfi Lima, Elizabeth Cirne |
author_facet | Terraciano, Paula Barros Garcez, Tuane Alves Berger, Markus Durli, Isabel Kuhl, Cristiana Palma Batista, Vitória de Oliveira Schneider, Raquel de Almeida Festa, Jaquelline Pilar, Emily Goulart, Marcela Ferreira, Charles Passos, Eduardo Pandolfi Lima, Elizabeth Cirne |
author_sort | Terraciano, Paula Barros |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to investigate the efficacy of protocols for mice ovary cryopreservation to compare the differences in Mouse Vasa Homologue expression (a germline cell marker) and ovarian viability after vitrification or slow freezing. METHODS: Female CF1 mice aged 40-45 days were randomly divided into three groups: Control, vitrification or slow freezing. Their ovaries were surgically removed, rinsed in saline solution and cryopreserved. For vitrification, we used a commercial protocol and for slow freeze, we used 1.5 M ethylene glycol (EG) as cryoprotectant. After that, the ovaries were processed for histological an immunohistochemical analysis, and counting of primordial, primary, pre-antral and antral follicles. RESULTS: No significant difference was found in the proportion of high-quality primordial, primary and pre-antral follicles after thawing/warming in the slow freezing and vitrification groups. The immunohistochemistry for MVH antibody demonstrated that the slow freeze group had a higher number of unmarked cells (p=0.012), indicating a harmful effect on the MVH expression in the ovarian tissue, where the cell structure is complex. CONCLUSION: Although both protocols indicated similar results in the histological analysis of follicular counts, the vitrification protocol was significantly better to preserve ovarian stem cells, an immature germ cell population. These cells are able to self-renew having regeneration potential, and may be effective for the treatment of ovarian failure and consequently infertility. |
format | Online Article Text |
id | pubmed-6993165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Brazilian Society of Assisted Reproduction |
record_format | MEDLINE/PubMed |
spelling | pubmed-69931652020-02-11 Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice Terraciano, Paula Barros Garcez, Tuane Alves Berger, Markus Durli, Isabel Kuhl, Cristiana Palma Batista, Vitória de Oliveira Schneider, Raquel de Almeida Festa, Jaquelline Pilar, Emily Goulart, Marcela Ferreira, Charles Passos, Eduardo Pandolfi Lima, Elizabeth Cirne JBRA Assist Reprod Original Article OBJECTIVE: The aim of this study was to investigate the efficacy of protocols for mice ovary cryopreservation to compare the differences in Mouse Vasa Homologue expression (a germline cell marker) and ovarian viability after vitrification or slow freezing. METHODS: Female CF1 mice aged 40-45 days were randomly divided into three groups: Control, vitrification or slow freezing. Their ovaries were surgically removed, rinsed in saline solution and cryopreserved. For vitrification, we used a commercial protocol and for slow freeze, we used 1.5 M ethylene glycol (EG) as cryoprotectant. After that, the ovaries were processed for histological an immunohistochemical analysis, and counting of primordial, primary, pre-antral and antral follicles. RESULTS: No significant difference was found in the proportion of high-quality primordial, primary and pre-antral follicles after thawing/warming in the slow freezing and vitrification groups. The immunohistochemistry for MVH antibody demonstrated that the slow freeze group had a higher number of unmarked cells (p=0.012), indicating a harmful effect on the MVH expression in the ovarian tissue, where the cell structure is complex. CONCLUSION: Although both protocols indicated similar results in the histological analysis of follicular counts, the vitrification protocol was significantly better to preserve ovarian stem cells, an immature germ cell population. These cells are able to self-renew having regeneration potential, and may be effective for the treatment of ovarian failure and consequently infertility. Brazilian Society of Assisted Reproduction 2020 /pmc/articles/PMC6993165/ /pubmed/31689043 http://dx.doi.org/10.5935/1518-0557.20190057 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Terraciano, Paula Barros Garcez, Tuane Alves Berger, Markus Durli, Isabel Kuhl, Cristiana Palma Batista, Vitória de Oliveira Schneider, Raquel de Almeida Festa, Jaquelline Pilar, Emily Goulart, Marcela Ferreira, Charles Passos, Eduardo Pandolfi Lima, Elizabeth Cirne Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title | Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title_full | Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title_fullStr | Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title_full_unstemmed | Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title_short | Ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in CF-1 mice |
title_sort | ovarian tissue vitrification is more efficient than slow freezing to preserve ovarian stem cells in cf-1 mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993165/ https://www.ncbi.nlm.nih.gov/pubmed/31689043 http://dx.doi.org/10.5935/1518-0557.20190057 |
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