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Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)

Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation me...

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Autores principales: Chen, Li, Liu, Liantao, Lu, Bin, Ma, Tongtong, Jiang, Dan, Li, Jin, Zhang, Ke, Sun, Hongchun, Zhang, Yongjiang, Bai, Zhiying, Li, Cundong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994006/
https://www.ncbi.nlm.nih.gov/pubmed/32004326
http://dx.doi.org/10.1371/journal.pone.0228241
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author Chen, Li
Liu, Liantao
Lu, Bin
Ma, Tongtong
Jiang, Dan
Li, Jin
Zhang, Ke
Sun, Hongchun
Zhang, Yongjiang
Bai, Zhiying
Li, Cundong
author_facet Chen, Li
Liu, Liantao
Lu, Bin
Ma, Tongtong
Jiang, Dan
Li, Jin
Zhang, Ke
Sun, Hongchun
Zhang, Yongjiang
Bai, Zhiying
Li, Cundong
author_sort Chen, Li
collection PubMed
description Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10–100 μM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H(2)O(2)), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na(+), Cl(-)). Meanwhile, the contents of melatonin, soluble proteins, and K(+) as well as the K(+)/Na(+) balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H(2)O(2), MDA, Na(+), and Cl(-). The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K(+)/Na(+) contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 μM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance.
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spelling pubmed-69940062020-02-20 Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.) Chen, Li Liu, Liantao Lu, Bin Ma, Tongtong Jiang, Dan Li, Jin Zhang, Ke Sun, Hongchun Zhang, Yongjiang Bai, Zhiying Li, Cundong PLoS One Research Article Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10–100 μM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H(2)O(2)), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na(+), Cl(-)). Meanwhile, the contents of melatonin, soluble proteins, and K(+) as well as the K(+)/Na(+) balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H(2)O(2), MDA, Na(+), and Cl(-). The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K(+)/Na(+) contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 μM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance. Public Library of Science 2020-01-31 /pmc/articles/PMC6994006/ /pubmed/32004326 http://dx.doi.org/10.1371/journal.pone.0228241 Text en © 2020 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Li
Liu, Liantao
Lu, Bin
Ma, Tongtong
Jiang, Dan
Li, Jin
Zhang, Ke
Sun, Hongchun
Zhang, Yongjiang
Bai, Zhiying
Li, Cundong
Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title_full Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title_fullStr Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title_full_unstemmed Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title_short Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)
title_sort exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (gossypium hirsutum l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994006/
https://www.ncbi.nlm.nih.gov/pubmed/32004326
http://dx.doi.org/10.1371/journal.pone.0228241
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