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qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes

Here, we describe an electrophoresis free assay for characterizing Tn5 transposomes fragmentation efficiency in a tagmentation reaction, in which double-stranded DNA is fragmented and tagged with adapter sequences. The assay uses plasmid DNA as a reference tagmentation substrate. Fragmentation effic...

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Detalles Bibliográficos
Autores principales:	Rykalina, Vera, Shadrin, Alexey, Lehrach, Hans, Borodina, Tatiana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994069/ https://www.ncbi.nlm.nih.gov/pubmed/32161784 http://dx.doi.org/10.1093/biomethods/bpx001

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