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PrimerPooler: automated primer pooling to prepare library for targeted sequencing
Targeted next-generation sequencing based on PCR amplification involves pooling of hundreds to thousands of primers, for preamplification and subsequent parallel single/multiplex PCR. It is often necessary to allocate the set of primers into subpools, a common issue being potential cross-hybridizati...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994079/ https://www.ncbi.nlm.nih.gov/pubmed/32161789 http://dx.doi.org/10.1093/biomethods/bpx006 |
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