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Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir
The significance of the integral membrane protein Niemann-Pick C1 (NPC1) in the ebolavirus entry process has been determined using various cell lines derived from humans, non-human primates and fruit bats. Fruit bats have long been purported as the potential reservoir host for ebolaviruses, however...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994141/ https://www.ncbi.nlm.nih.gov/pubmed/31961874 http://dx.doi.org/10.1371/journal.pntd.0007952 |
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author | Bokelmann, Marcel Edenborough, Kathryn Hetzelt, Nicole Kreher, Petra Lander, Angelika Nitsche, Andreas Vogel, Uwe Feldmann, Heinz Couacy-Hymann, Emmanuel Kurth, Andreas |
author_facet | Bokelmann, Marcel Edenborough, Kathryn Hetzelt, Nicole Kreher, Petra Lander, Angelika Nitsche, Andreas Vogel, Uwe Feldmann, Heinz Couacy-Hymann, Emmanuel Kurth, Andreas |
author_sort | Bokelmann, Marcel |
collection | PubMed |
description | The significance of the integral membrane protein Niemann-Pick C1 (NPC1) in the ebolavirus entry process has been determined using various cell lines derived from humans, non-human primates and fruit bats. Fruit bats have long been purported as the potential reservoir host for ebolaviruses, however several studies provide evidence that Mops condylurus, an insectivorous microbat, is also an ebolavirus reservoir. NPC1 receptor expression in the context of ebolavirus replication in microbat cells remains unstudied. In order to study Ebola virus (EBOV) cellular entry and replication in M. condylurus, we derived primary and immortalized cell cultures from 12 different organs. The NPC1 receptor expression was characterized by confocal microscopy and flow cytometry comparing the expression levels of M. condylurus primary and immortalized cells, HeLa cells, human embryonic kidney cells and cells from a European microbat species. EBOV replication kinetics was studied for four representative cell cultures using qRT-PCR. The aim was to elucidate the suitability of primary and immortalized cells from different tissues for studying NPC1 receptor expression levels and their potential influence on EBOV replication. The NPC1 receptor expression level in M. condylurus primary cells differed depending on the organ they were derived from and was for most cell types significantly lower than in human cell lines. Immortalized cells showed for most cell types higher expression levels than their corresponding primary cells. Concluding from our infection experiments with EBOV we suggest a potential correlation between NPC1 receptor expression level and virus replication rate in vitro. |
format | Online Article Text |
id | pubmed-6994141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69941412020-02-18 Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir Bokelmann, Marcel Edenborough, Kathryn Hetzelt, Nicole Kreher, Petra Lander, Angelika Nitsche, Andreas Vogel, Uwe Feldmann, Heinz Couacy-Hymann, Emmanuel Kurth, Andreas PLoS Negl Trop Dis Research Article The significance of the integral membrane protein Niemann-Pick C1 (NPC1) in the ebolavirus entry process has been determined using various cell lines derived from humans, non-human primates and fruit bats. Fruit bats have long been purported as the potential reservoir host for ebolaviruses, however several studies provide evidence that Mops condylurus, an insectivorous microbat, is also an ebolavirus reservoir. NPC1 receptor expression in the context of ebolavirus replication in microbat cells remains unstudied. In order to study Ebola virus (EBOV) cellular entry and replication in M. condylurus, we derived primary and immortalized cell cultures from 12 different organs. The NPC1 receptor expression was characterized by confocal microscopy and flow cytometry comparing the expression levels of M. condylurus primary and immortalized cells, HeLa cells, human embryonic kidney cells and cells from a European microbat species. EBOV replication kinetics was studied for four representative cell cultures using qRT-PCR. The aim was to elucidate the suitability of primary and immortalized cells from different tissues for studying NPC1 receptor expression levels and their potential influence on EBOV replication. The NPC1 receptor expression level in M. condylurus primary cells differed depending on the organ they were derived from and was for most cell types significantly lower than in human cell lines. Immortalized cells showed for most cell types higher expression levels than their corresponding primary cells. Concluding from our infection experiments with EBOV we suggest a potential correlation between NPC1 receptor expression level and virus replication rate in vitro. Public Library of Science 2020-01-21 /pmc/articles/PMC6994141/ /pubmed/31961874 http://dx.doi.org/10.1371/journal.pntd.0007952 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Bokelmann, Marcel Edenborough, Kathryn Hetzelt, Nicole Kreher, Petra Lander, Angelika Nitsche, Andreas Vogel, Uwe Feldmann, Heinz Couacy-Hymann, Emmanuel Kurth, Andreas Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title | Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title_full | Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title_fullStr | Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title_full_unstemmed | Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title_short | Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir |
title_sort | utility of primary cells to examine npc1 receptor expression in mops condylurus, a potential ebola virus reservoir |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994141/ https://www.ncbi.nlm.nih.gov/pubmed/31961874 http://dx.doi.org/10.1371/journal.pntd.0007952 |
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