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Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy
Microfluidic chips provide a powerful platform for high-throughput screening of diverse biophysical systems. The most prevalent detection methods are fluorescence based. Developing new readout techniques for microfluidics focusing on quantitative information in the low signal regime is desirable. In...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994618/ https://www.ncbi.nlm.nih.gov/pubmed/32005843 http://dx.doi.org/10.1038/s41598-020-58307-w |
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author | Wissberg, Shai Ronen, Maria Oren, Ziv Gerber, Doron Kalisky, Beena |
author_facet | Wissberg, Shai Ronen, Maria Oren, Ziv Gerber, Doron Kalisky, Beena |
author_sort | Wissberg, Shai |
collection | PubMed |
description | Microfluidic chips provide a powerful platform for high-throughput screening of diverse biophysical systems. The most prevalent detection methods are fluorescence based. Developing new readout techniques for microfluidics focusing on quantitative information in the low signal regime is desirable. In this work, we combine the well-established immunoassay approach, with magnetic nanoparticles, with a highly sensitive magnetic imaging technique. We offer to integrate a microfluidic array into a scanning superconducting quantum interference device (SQUID) microscope, to image nanoparticles that were moved through the microfluidic device. We demonstrate the technique on protein-protein interactions (PPI). We compare sensitivity to that of a conventional readout, quantify the amount of interactions, and demonstrate 0.1 atto-mole sensitivity. Our work serves as a proof of concept that will promote the development of a new set of eyes, a stable usable microfluidic-scanning SQUID microscopy. |
format | Online Article Text |
id | pubmed-6994618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69946182020-02-06 Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy Wissberg, Shai Ronen, Maria Oren, Ziv Gerber, Doron Kalisky, Beena Sci Rep Article Microfluidic chips provide a powerful platform for high-throughput screening of diverse biophysical systems. The most prevalent detection methods are fluorescence based. Developing new readout techniques for microfluidics focusing on quantitative information in the low signal regime is desirable. In this work, we combine the well-established immunoassay approach, with magnetic nanoparticles, with a highly sensitive magnetic imaging technique. We offer to integrate a microfluidic array into a scanning superconducting quantum interference device (SQUID) microscope, to image nanoparticles that were moved through the microfluidic device. We demonstrate the technique on protein-protein interactions (PPI). We compare sensitivity to that of a conventional readout, quantify the amount of interactions, and demonstrate 0.1 atto-mole sensitivity. Our work serves as a proof of concept that will promote the development of a new set of eyes, a stable usable microfluidic-scanning SQUID microscopy. Nature Publishing Group UK 2020-01-31 /pmc/articles/PMC6994618/ /pubmed/32005843 http://dx.doi.org/10.1038/s41598-020-58307-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wissberg, Shai Ronen, Maria Oren, Ziv Gerber, Doron Kalisky, Beena Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title | Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title_full | Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title_fullStr | Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title_full_unstemmed | Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title_short | Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy |
title_sort | sensitive readout for microfluidic high-throughput applications using scanning squid microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994618/ https://www.ncbi.nlm.nih.gov/pubmed/32005843 http://dx.doi.org/10.1038/s41598-020-58307-w |
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