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Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36
Grey mould is an important necrotrophic fungal pathogen that causes huge economic losses in agriculture. Many types of bacteria are used for biological control of grey mould via competition for space or nutrients and/or the production of antifungal metabolites. Oxalate is a key component of virulent...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994688/ https://www.ncbi.nlm.nih.gov/pubmed/32005892 http://dx.doi.org/10.1038/s41598-020-58609-z |
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author | Lee, Yeyeong Choi, Okhee Kang, Byeongsam Bae, Juyoung Kim, Seunghoe Kim, Jinwoo |
author_facet | Lee, Yeyeong Choi, Okhee Kang, Byeongsam Bae, Juyoung Kim, Seunghoe Kim, Jinwoo |
author_sort | Lee, Yeyeong |
collection | PubMed |
description | Grey mould is an important necrotrophic fungal pathogen that causes huge economic losses in agriculture. Many types of bacteria are used for biological control of grey mould via competition for space or nutrients and/or the production of antifungal metabolites. Oxalate is a key component of virulent necrotic fungal pathogens. In this study, we isolated non-antifungal oxalate-degrading bacteria (ODB) from the surfaces of oxalate-rich spinach and strawberries to investigate their ability to control necrotic fungal pathogens such as grey mould. A total of 36 bacteria grown on oxalate minimal (OM) agar plates were tested for oxalate-degrading activity. Five isolates exhibiting the highest oxalate degradation activity were subjected to molecular identification using 16S rRNA gene sequencing. Two isolates exhibiting non-antifungal activity were subjected to disease suppression assays using Arabidopsis–Botrytis systems. The isolate Pseudomonas abietaniphila ODB36, which exhibited significant plant protective ability, was finally selected for further investigation. Based on whole-genome information, the pseudomonad oxalate degrading (podA) gene, which encodes formyl-CoA transferase, was analysed. The podA(−) mutant did not inhibit Botrytis infection and oxalate toxicity; the defects were recovered by podA complementation. Purified PodA–His converted oxalate to formate and eliminated oxalate toxicity. These results indicate that P. abietaniphila ODB36 and PodA enzyme are associated with various aspects of grey mould disease inhibitory effects. |
format | Online Article Text |
id | pubmed-6994688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69946882020-02-06 Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 Lee, Yeyeong Choi, Okhee Kang, Byeongsam Bae, Juyoung Kim, Seunghoe Kim, Jinwoo Sci Rep Article Grey mould is an important necrotrophic fungal pathogen that causes huge economic losses in agriculture. Many types of bacteria are used for biological control of grey mould via competition for space or nutrients and/or the production of antifungal metabolites. Oxalate is a key component of virulent necrotic fungal pathogens. In this study, we isolated non-antifungal oxalate-degrading bacteria (ODB) from the surfaces of oxalate-rich spinach and strawberries to investigate their ability to control necrotic fungal pathogens such as grey mould. A total of 36 bacteria grown on oxalate minimal (OM) agar plates were tested for oxalate-degrading activity. Five isolates exhibiting the highest oxalate degradation activity were subjected to molecular identification using 16S rRNA gene sequencing. Two isolates exhibiting non-antifungal activity were subjected to disease suppression assays using Arabidopsis–Botrytis systems. The isolate Pseudomonas abietaniphila ODB36, which exhibited significant plant protective ability, was finally selected for further investigation. Based on whole-genome information, the pseudomonad oxalate degrading (podA) gene, which encodes formyl-CoA transferase, was analysed. The podA(−) mutant did not inhibit Botrytis infection and oxalate toxicity; the defects were recovered by podA complementation. Purified PodA–His converted oxalate to formate and eliminated oxalate toxicity. These results indicate that P. abietaniphila ODB36 and PodA enzyme are associated with various aspects of grey mould disease inhibitory effects. Nature Publishing Group UK 2020-01-31 /pmc/articles/PMC6994688/ /pubmed/32005892 http://dx.doi.org/10.1038/s41598-020-58609-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, Yeyeong Choi, Okhee Kang, Byeongsam Bae, Juyoung Kim, Seunghoe Kim, Jinwoo Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title | Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title_full | Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title_fullStr | Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title_full_unstemmed | Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title_short | Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 |
title_sort | grey mould control by oxalate degradation using non-antifungal pseudomonas abietaniphila strain odb36 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994688/ https://www.ncbi.nlm.nih.gov/pubmed/32005892 http://dx.doi.org/10.1038/s41598-020-58609-z |
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