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Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants
The photosynthetic apparatus of higher plants can dissipate excess excitation energy during high light exposure, by deactivating excited chlorophylls through a mechanism called nonphotochemical quenching (NPQ). However, the precise molecular details of quenching and the mechanism regulating the quen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994720/ https://www.ncbi.nlm.nih.gov/pubmed/32005811 http://dx.doi.org/10.1038/s41467-020-14488-6 |
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author | Cupellini, Lorenzo Calvani, Dario Jacquemin, Denis Mennucci, Benedetta |
author_facet | Cupellini, Lorenzo Calvani, Dario Jacquemin, Denis Mennucci, Benedetta |
author_sort | Cupellini, Lorenzo |
collection | PubMed |
description | The photosynthetic apparatus of higher plants can dissipate excess excitation energy during high light exposure, by deactivating excited chlorophylls through a mechanism called nonphotochemical quenching (NPQ). However, the precise molecular details of quenching and the mechanism regulating the quenching level are still not completely understood. Focusing on the major light-harvesting complex LHCII of Photosystem II, we show that a charge transfer state involving Lutein can efficiently quench chlorophyll excitation, and reduce the excitation lifetime of LHCII to the levels measured in the deeply quenched LHCII aggregates. Through a combination of molecular dynamics simulations, multiscale quantum chemical calculations, and kinetic modeling, we demonstrate that the quenching level can be finely tuned by the protein, by regulating the energy of the charge transfer state. Our results suggest that a limited conformational rearrangement of the protein scaffold could act as a molecular switch to activate or deactivate the quenching mechanism. |
format | Online Article Text |
id | pubmed-6994720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69947202020-02-03 Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants Cupellini, Lorenzo Calvani, Dario Jacquemin, Denis Mennucci, Benedetta Nat Commun Article The photosynthetic apparatus of higher plants can dissipate excess excitation energy during high light exposure, by deactivating excited chlorophylls through a mechanism called nonphotochemical quenching (NPQ). However, the precise molecular details of quenching and the mechanism regulating the quenching level are still not completely understood. Focusing on the major light-harvesting complex LHCII of Photosystem II, we show that a charge transfer state involving Lutein can efficiently quench chlorophyll excitation, and reduce the excitation lifetime of LHCII to the levels measured in the deeply quenched LHCII aggregates. Through a combination of molecular dynamics simulations, multiscale quantum chemical calculations, and kinetic modeling, we demonstrate that the quenching level can be finely tuned by the protein, by regulating the energy of the charge transfer state. Our results suggest that a limited conformational rearrangement of the protein scaffold could act as a molecular switch to activate or deactivate the quenching mechanism. Nature Publishing Group UK 2020-01-31 /pmc/articles/PMC6994720/ /pubmed/32005811 http://dx.doi.org/10.1038/s41467-020-14488-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Cupellini, Lorenzo Calvani, Dario Jacquemin, Denis Mennucci, Benedetta Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title | Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title_full | Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title_fullStr | Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title_full_unstemmed | Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title_short | Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
title_sort | charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994720/ https://www.ncbi.nlm.nih.gov/pubmed/32005811 http://dx.doi.org/10.1038/s41467-020-14488-6 |
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