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Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus
BACKGROUND: Quantitative PCR (qPCR) is a powerful tool that is particularly well-suited to measure mRNA levels in clinical samples, especially those with relatively low cell counts. However, a caveat of this approach is that reliable, stably expressed reference (housekeeping) genes are vital in orde...
Autores principales: | Roy, Justin G., McElhaney, Janet E., Verschoor, Chris P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995044/ https://www.ncbi.nlm.nih.gov/pubmed/32005148 http://dx.doi.org/10.1186/s12865-020-0334-8 |
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