Cargando…
MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1
Prostate cancer (PCa) is a heterogeneous malignancy, and is a primary cause of cancer-related death in males. Forkhead box transcription factor O1 (FOXO1) exerts antitumor effects in various cancers, including PCa. However, the regulatory mechanism of miR-142-3p on FOXO1 expression in human PCa has...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995382/ https://www.ncbi.nlm.nih.gov/pubmed/32047567 http://dx.doi.org/10.7150/jca.41888 |
_version_ | 1783493370966966272 |
---|---|
author | Tan, Yi-Fan Chen, Zhi-Yuan Wang, Lei Wang, Min Liu, Xiu-Heng |
author_facet | Tan, Yi-Fan Chen, Zhi-Yuan Wang, Lei Wang, Min Liu, Xiu-Heng |
author_sort | Tan, Yi-Fan |
collection | PubMed |
description | Prostate cancer (PCa) is a heterogeneous malignancy, and is a primary cause of cancer-related death in males. Forkhead box transcription factor O1 (FOXO1) exerts antitumor effects in various cancers, including PCa. However, the regulatory mechanism of miR-142-3p on FOXO1 expression in human PCa has not been characterized. In this study, we showed that FOXO1 protein levels were downregulated in PCa tissues and cells. Moreover, FOXO1 expression was a predictor of disease-free survival in patients with PCa and was a predictor of prognosis. Increased expression of FOXO1 suppressed cellular proliferation and induced cell cycle arrest at G0/G1 in vitro. However, FOXO1 mRNA and protein levels were inconsistent in human PCa tissues and cell lines. We showed that miR-142-3p levels were negatively correlated with FOXO1 protein levels in PCa. We also showed that miR-142-3p suppressed FOXO1 expression by directly targeting its 3′-untranslated region. Furthermore, suppression of miR-142-3p inhibited cell proliferation and induced cell cycle arrest, and these effects were blocked by FOXO1 knockdown. In vivo experiments showed that miR-142-3p knockout impaired tumor growth. Our results validate that FOXO1 acted as a tumor suppressor in PCa and demonstrated that FOXO1 was regulated by miR-142-3p, and miR-142-3p may be a potential target for treatment of PCa. |
format | Online Article Text |
id | pubmed-6995382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-69953822020-02-11 MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 Tan, Yi-Fan Chen, Zhi-Yuan Wang, Lei Wang, Min Liu, Xiu-Heng J Cancer Research Paper Prostate cancer (PCa) is a heterogeneous malignancy, and is a primary cause of cancer-related death in males. Forkhead box transcription factor O1 (FOXO1) exerts antitumor effects in various cancers, including PCa. However, the regulatory mechanism of miR-142-3p on FOXO1 expression in human PCa has not been characterized. In this study, we showed that FOXO1 protein levels were downregulated in PCa tissues and cells. Moreover, FOXO1 expression was a predictor of disease-free survival in patients with PCa and was a predictor of prognosis. Increased expression of FOXO1 suppressed cellular proliferation and induced cell cycle arrest at G0/G1 in vitro. However, FOXO1 mRNA and protein levels were inconsistent in human PCa tissues and cell lines. We showed that miR-142-3p levels were negatively correlated with FOXO1 protein levels in PCa. We also showed that miR-142-3p suppressed FOXO1 expression by directly targeting its 3′-untranslated region. Furthermore, suppression of miR-142-3p inhibited cell proliferation and induced cell cycle arrest, and these effects were blocked by FOXO1 knockdown. In vivo experiments showed that miR-142-3p knockout impaired tumor growth. Our results validate that FOXO1 acted as a tumor suppressor in PCa and demonstrated that FOXO1 was regulated by miR-142-3p, and miR-142-3p may be a potential target for treatment of PCa. Ivyspring International Publisher 2020-01-14 /pmc/articles/PMC6995382/ /pubmed/32047567 http://dx.doi.org/10.7150/jca.41888 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Tan, Yi-Fan Chen, Zhi-Yuan Wang, Lei Wang, Min Liu, Xiu-Heng MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title | MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title_full | MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title_fullStr | MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title_full_unstemmed | MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title_short | MiR-142-3p functions as an oncogene in prostate cancer by targeting FOXO1 |
title_sort | mir-142-3p functions as an oncogene in prostate cancer by targeting foxo1 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995382/ https://www.ncbi.nlm.nih.gov/pubmed/32047567 http://dx.doi.org/10.7150/jca.41888 |
work_keys_str_mv | AT tanyifan mir1423pfunctionsasanoncogeneinprostatecancerbytargetingfoxo1 AT chenzhiyuan mir1423pfunctionsasanoncogeneinprostatecancerbytargetingfoxo1 AT wanglei mir1423pfunctionsasanoncogeneinprostatecancerbytargetingfoxo1 AT wangmin mir1423pfunctionsasanoncogeneinprostatecancerbytargetingfoxo1 AT liuxiuheng mir1423pfunctionsasanoncogeneinprostatecancerbytargetingfoxo1 |