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N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats
Since cisplatin therapy is usually accompanied with numerous toxicities, including neurotoxicity, that involve tissue oxidative damage, the aim of this study was to evaluate the possible protective effect of N-acetylcysteine (NAC) on the anxiogenic response to cisplatin (CIS). Thirty-two male Wistar...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995611/ https://www.ncbi.nlm.nih.gov/pubmed/31861240 http://dx.doi.org/10.3390/biom9120892 |
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author | Vukovic, Rade Kumburovic, Igor Joksimovic Jovic, Jovana Jovicic, Nemanja Katanic Stankovic, Jelena S. Mihailovic, Vladimir Djuric, Milos Velickovic, Stefan Arnaut, Aleksandra Selakovic, Dragica Rosic, Gvozden |
author_facet | Vukovic, Rade Kumburovic, Igor Joksimovic Jovic, Jovana Jovicic, Nemanja Katanic Stankovic, Jelena S. Mihailovic, Vladimir Djuric, Milos Velickovic, Stefan Arnaut, Aleksandra Selakovic, Dragica Rosic, Gvozden |
author_sort | Vukovic, Rade |
collection | PubMed |
description | Since cisplatin therapy is usually accompanied with numerous toxicities, including neurotoxicity, that involve tissue oxidative damage, the aim of this study was to evaluate the possible protective effect of N-acetylcysteine (NAC) on the anxiogenic response to cisplatin (CIS). Thirty-two male Wistar albino rats divided into four groups (control, cisplatin, NAC, and CIS + NAC). All treatments were delivered intraperitoneally. On day one, the control and cisplatin groups received saline while the NAC and CIS + NAC groups were administered with NAC (500 mg/kg). On the fifth day, the control group received saline while the CIS group was treated with cisplatin (7.5 mg/kg), the NAC group again received NAC (500 mg/kg), and the CIS + NAC group was simultaneously treated with cisplatin and NAC (7.5 and 500 mg/kg, respectively). Behavioral testing, performed on the tenth day in the open field (OF) and elevated plus maze (EPM) tests, revealed the anxiogenic effect of cisplatin that was significantly attenuated by NAC. The hippocampal sections evaluation showed increased oxidative stress (increased lipid peroxidation and decline in antioxidant enzymes activity) and proapoptotic action (predominantly by diminished antiapoptotic gene expression) following a single dose of cisplatin. NAC supplementation along with cisplatin administration reversed the prooxidative and proapoptotic effects of cisplatin. In conclusion, the results obtained in this study confirmed that antioxidant supplementation with NAC may attenuate the cisplatin-induced anxiety. The mechanism of anxiolytic effect achieved by NAC may include the decline in oxidative damage that down regulates increased apoptosis and reverses the anxiogenic action of cisplatin. |
format | Online Article Text |
id | pubmed-6995611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69956112020-02-13 N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats Vukovic, Rade Kumburovic, Igor Joksimovic Jovic, Jovana Jovicic, Nemanja Katanic Stankovic, Jelena S. Mihailovic, Vladimir Djuric, Milos Velickovic, Stefan Arnaut, Aleksandra Selakovic, Dragica Rosic, Gvozden Biomolecules Article Since cisplatin therapy is usually accompanied with numerous toxicities, including neurotoxicity, that involve tissue oxidative damage, the aim of this study was to evaluate the possible protective effect of N-acetylcysteine (NAC) on the anxiogenic response to cisplatin (CIS). Thirty-two male Wistar albino rats divided into four groups (control, cisplatin, NAC, and CIS + NAC). All treatments were delivered intraperitoneally. On day one, the control and cisplatin groups received saline while the NAC and CIS + NAC groups were administered with NAC (500 mg/kg). On the fifth day, the control group received saline while the CIS group was treated with cisplatin (7.5 mg/kg), the NAC group again received NAC (500 mg/kg), and the CIS + NAC group was simultaneously treated with cisplatin and NAC (7.5 and 500 mg/kg, respectively). Behavioral testing, performed on the tenth day in the open field (OF) and elevated plus maze (EPM) tests, revealed the anxiogenic effect of cisplatin that was significantly attenuated by NAC. The hippocampal sections evaluation showed increased oxidative stress (increased lipid peroxidation and decline in antioxidant enzymes activity) and proapoptotic action (predominantly by diminished antiapoptotic gene expression) following a single dose of cisplatin. NAC supplementation along with cisplatin administration reversed the prooxidative and proapoptotic effects of cisplatin. In conclusion, the results obtained in this study confirmed that antioxidant supplementation with NAC may attenuate the cisplatin-induced anxiety. The mechanism of anxiolytic effect achieved by NAC may include the decline in oxidative damage that down regulates increased apoptosis and reverses the anxiogenic action of cisplatin. MDPI 2019-12-17 /pmc/articles/PMC6995611/ /pubmed/31861240 http://dx.doi.org/10.3390/biom9120892 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vukovic, Rade Kumburovic, Igor Joksimovic Jovic, Jovana Jovicic, Nemanja Katanic Stankovic, Jelena S. Mihailovic, Vladimir Djuric, Milos Velickovic, Stefan Arnaut, Aleksandra Selakovic, Dragica Rosic, Gvozden N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title | N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title_full | N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title_fullStr | N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title_full_unstemmed | N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title_short | N-Acetylcysteine Protects against the Anxiogenic Response to Cisplatin in Rats |
title_sort | n-acetylcysteine protects against the anxiogenic response to cisplatin in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995611/ https://www.ncbi.nlm.nih.gov/pubmed/31861240 http://dx.doi.org/10.3390/biom9120892 |
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