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Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive

BACKGROUND: Verticillium wilt of olive (VWO) is caused by the soilborne fungal pathogen Verticillium dahliae. One of the best VWO management measures is the use of tolerant/resistant olive cultivars. Knowledge on the olive-associated microbiome and its potential relationship with tolerance to biotic...

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Autores principales: Fernández-González, Antonio J., Cardoni, Martina, Gómez-Lama Cabanás, Carmen, Valverde-Corredor, Antonio, Villadas, Pablo J., Fernández-López, Manuel, Mercado-Blanco, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995654/
https://www.ncbi.nlm.nih.gov/pubmed/32007096
http://dx.doi.org/10.1186/s40168-020-0787-2
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author Fernández-González, Antonio J.
Cardoni, Martina
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
author_facet Fernández-González, Antonio J.
Cardoni, Martina
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
author_sort Fernández-González, Antonio J.
collection PubMed
description BACKGROUND: Verticillium wilt of olive (VWO) is caused by the soilborne fungal pathogen Verticillium dahliae. One of the best VWO management measures is the use of tolerant/resistant olive cultivars. Knowledge on the olive-associated microbiome and its potential relationship with tolerance to biotic constraints is almost null. The aims of this work are (1) to describe the structure, functionality, and co-occurrence interactions of the belowground (root endosphere and rhizosphere) microbial communities of two olive cultivars qualified as tolerant (Frantoio) and susceptible (Picual) to VWO, and (2) to assess whether these communities contribute to their differential disease susceptibility level. RESULTS: Minor differences in alpha and beta diversities of root-associated microbiota were detected between olive cultivars regardless of whether they were inoculated or not with the defoliating pathotype of V. dahliae. Nevertheless, significant differences were found in taxonomic composition of non-inoculated plants’ communities, “Frantoio” showing a higher abundance of beneficial genera in contrast to “Picual” that exhibited major abundance of potential deleterious genera. Upon inoculation with V. dahliae, significant changes at taxonomic level were found mostly in Picual plants. Relevant topological alterations were observed in microbial communities’ co-occurrence interactions after inoculation, both at structural and functional level, and in the positive/negative edges ratio. In the root endosphere, Frantoio communities switched to highly connected and low modularized networks, while Picual communities showed a sharply different behavior. In the rhizosphere, V. dahliae only irrupted in the microbial networks of Picual plants. CONCLUSIONS: The belowground microbial communities of the two olive cultivars are very similar and pathogen introduction did not provoke significant alterations in their structure and functionality. However, notable differences were found in their networks in response to the inoculation. This phenomenon was more evident in the root endosphere communities. Thus, a correlation between modifications in the microbial networks of this microhabitat and susceptibility/tolerance to a soilborne pathogen was found. Moreover, V. dahliae irruption in the Picual microbial networks suggests a stronger impact on the belowground microbial communities of this cultivar upon inoculation. Our results suggest that changes in the co-occurrence interactions may explain, at least partially, the differential VWO susceptibility of the tested olive cultivars.
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spelling pubmed-69956542020-02-05 Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive Fernández-González, Antonio J. Cardoni, Martina Gómez-Lama Cabanás, Carmen Valverde-Corredor, Antonio Villadas, Pablo J. Fernández-López, Manuel Mercado-Blanco, Jesús Microbiome Research BACKGROUND: Verticillium wilt of olive (VWO) is caused by the soilborne fungal pathogen Verticillium dahliae. One of the best VWO management measures is the use of tolerant/resistant olive cultivars. Knowledge on the olive-associated microbiome and its potential relationship with tolerance to biotic constraints is almost null. The aims of this work are (1) to describe the structure, functionality, and co-occurrence interactions of the belowground (root endosphere and rhizosphere) microbial communities of two olive cultivars qualified as tolerant (Frantoio) and susceptible (Picual) to VWO, and (2) to assess whether these communities contribute to their differential disease susceptibility level. RESULTS: Minor differences in alpha and beta diversities of root-associated microbiota were detected between olive cultivars regardless of whether they were inoculated or not with the defoliating pathotype of V. dahliae. Nevertheless, significant differences were found in taxonomic composition of non-inoculated plants’ communities, “Frantoio” showing a higher abundance of beneficial genera in contrast to “Picual” that exhibited major abundance of potential deleterious genera. Upon inoculation with V. dahliae, significant changes at taxonomic level were found mostly in Picual plants. Relevant topological alterations were observed in microbial communities’ co-occurrence interactions after inoculation, both at structural and functional level, and in the positive/negative edges ratio. In the root endosphere, Frantoio communities switched to highly connected and low modularized networks, while Picual communities showed a sharply different behavior. In the rhizosphere, V. dahliae only irrupted in the microbial networks of Picual plants. CONCLUSIONS: The belowground microbial communities of the two olive cultivars are very similar and pathogen introduction did not provoke significant alterations in their structure and functionality. However, notable differences were found in their networks in response to the inoculation. This phenomenon was more evident in the root endosphere communities. Thus, a correlation between modifications in the microbial networks of this microhabitat and susceptibility/tolerance to a soilborne pathogen was found. Moreover, V. dahliae irruption in the Picual microbial networks suggests a stronger impact on the belowground microbial communities of this cultivar upon inoculation. Our results suggest that changes in the co-occurrence interactions may explain, at least partially, the differential VWO susceptibility of the tested olive cultivars. BioMed Central 2020-02-01 /pmc/articles/PMC6995654/ /pubmed/32007096 http://dx.doi.org/10.1186/s40168-020-0787-2 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Fernández-González, Antonio J.
Cardoni, Martina
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title_full Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title_fullStr Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title_full_unstemmed Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title_short Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive
title_sort linking belowground microbial network changes to different tolerance level towards verticillium wilt of olive
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995654/
https://www.ncbi.nlm.nih.gov/pubmed/32007096
http://dx.doi.org/10.1186/s40168-020-0787-2
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