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Perspective review of optical imaging in welfare assessment in animal-based research
To refine animal research, vital signs, activity, stress, and pain must be monitored. In chronic studies, some measures can be assessed using telemetry sensors. Although this methodology provides high-precision data, an initial surgery for device implantation is necessary, potentially leading to str...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995877/ https://www.ncbi.nlm.nih.gov/pubmed/31286726 http://dx.doi.org/10.1117/1.JBO.24.7.070601 |
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author | Pereira, Carina Barbosa Kunczik, Janosch Bleich, André Haeger, Christine Kiessling, Fabian Thum, Thomas Tolba, René Lindauer, Ute Treue, Stefan Czaplik, Michael |
author_facet | Pereira, Carina Barbosa Kunczik, Janosch Bleich, André Haeger, Christine Kiessling, Fabian Thum, Thomas Tolba, René Lindauer, Ute Treue, Stefan Czaplik, Michael |
author_sort | Pereira, Carina Barbosa |
collection | PubMed |
description | To refine animal research, vital signs, activity, stress, and pain must be monitored. In chronic studies, some measures can be assessed using telemetry sensors. Although this methodology provides high-precision data, an initial surgery for device implantation is necessary, potentially leading to stress, wound infections, and restriction of motion. Recently, camera systems have been adapted for animal research. We give an overview of parameters that can be assessed using imaging in the visible, near-infrared, and thermal spectrum of light. It focuses on heart activity, respiration, oxygen saturation, and motion, as well as on wound analysis. For each parameter, we offer recommendations on the minimum technical requirements of appropriate systems, regions of interest, and light conditions, among others. In general, these systems demonstrate great performance. For heart and respiratory rate, the error was [Formula: see text] and 5 breaths/min. Furthermore, the systems are capable of tracking animals during different behavioral tasks. Finally, studies indicate that inhomogeneous temperature distribution around wounds might be an indicator of (pending) infections. In sum, camera-based techniques have several applications in animal research. As vital parameters are currently only assessed in sedated animals, the next step should be the integration of these modalities in home-cage monitoring. |
format | Online Article Text |
id | pubmed-6995877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-69958772020-02-10 Perspective review of optical imaging in welfare assessment in animal-based research Pereira, Carina Barbosa Kunczik, Janosch Bleich, André Haeger, Christine Kiessling, Fabian Thum, Thomas Tolba, René Lindauer, Ute Treue, Stefan Czaplik, Michael J Biomed Opt Perspectives To refine animal research, vital signs, activity, stress, and pain must be monitored. In chronic studies, some measures can be assessed using telemetry sensors. Although this methodology provides high-precision data, an initial surgery for device implantation is necessary, potentially leading to stress, wound infections, and restriction of motion. Recently, camera systems have been adapted for animal research. We give an overview of parameters that can be assessed using imaging in the visible, near-infrared, and thermal spectrum of light. It focuses on heart activity, respiration, oxygen saturation, and motion, as well as on wound analysis. For each parameter, we offer recommendations on the minimum technical requirements of appropriate systems, regions of interest, and light conditions, among others. In general, these systems demonstrate great performance. For heart and respiratory rate, the error was [Formula: see text] and 5 breaths/min. Furthermore, the systems are capable of tracking animals during different behavioral tasks. Finally, studies indicate that inhomogeneous temperature distribution around wounds might be an indicator of (pending) infections. In sum, camera-based techniques have several applications in animal research. As vital parameters are currently only assessed in sedated animals, the next step should be the integration of these modalities in home-cage monitoring. Society of Photo-Optical Instrumentation Engineers 2019-07-08 2019-07 /pmc/articles/PMC6995877/ /pubmed/31286726 http://dx.doi.org/10.1117/1.JBO.24.7.070601 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Perspectives Pereira, Carina Barbosa Kunczik, Janosch Bleich, André Haeger, Christine Kiessling, Fabian Thum, Thomas Tolba, René Lindauer, Ute Treue, Stefan Czaplik, Michael Perspective review of optical imaging in welfare assessment in animal-based research |
title | Perspective review of optical imaging in welfare assessment in animal-based research |
title_full | Perspective review of optical imaging in welfare assessment in animal-based research |
title_fullStr | Perspective review of optical imaging in welfare assessment in animal-based research |
title_full_unstemmed | Perspective review of optical imaging in welfare assessment in animal-based research |
title_short | Perspective review of optical imaging in welfare assessment in animal-based research |
title_sort | perspective review of optical imaging in welfare assessment in animal-based research |
topic | Perspectives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995877/ https://www.ncbi.nlm.nih.gov/pubmed/31286726 http://dx.doi.org/10.1117/1.JBO.24.7.070601 |
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