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Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices

Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar...

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Autores principales: Hammad, S. J., Cavallero, S., Al-Nasiri, F. S., Amelio, S. D᾿
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996253/
https://www.ncbi.nlm.nih.gov/pubmed/32063742
http://dx.doi.org/10.2478/helm-2020-0010
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author Hammad, S. J.
Cavallero, S.
Al-Nasiri, F. S.
Amelio, S. D᾿
author_facet Hammad, S. J.
Cavallero, S.
Al-Nasiri, F. S.
Amelio, S. D᾿
author_sort Hammad, S. J.
collection PubMed
description Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue).
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spelling pubmed-69962532020-02-14 Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices Hammad, S. J. Cavallero, S. Al-Nasiri, F. S. Amelio, S. D᾿ Helminthologia Research Note Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue). Sciendo 2020-01-25 /pmc/articles/PMC6996253/ /pubmed/32063742 http://dx.doi.org/10.2478/helm-2020-0010 Text en © 2020 S. J. Hammad, S. Cavallero, F. S. Al-Nasiri, S. D᾿Amelio, published by Sciendo http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
spellingShingle Research Note
Hammad, S. J.
Cavallero, S.
Al-Nasiri, F. S.
Amelio, S. D᾿
Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title_full Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title_fullStr Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title_full_unstemmed Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title_short Comparative Study Among Lactophenol Blue, Lactophenol Solution and Proteinase-K Lytic Solution for Rostellar Hooks Morphometry of Echinococcus Granulosus Protoscolices
title_sort comparative study among lactophenol blue, lactophenol solution and proteinase-k lytic solution for rostellar hooks morphometry of echinococcus granulosus protoscolices
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996253/
https://www.ncbi.nlm.nih.gov/pubmed/32063742
http://dx.doi.org/10.2478/helm-2020-0010
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