An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies

During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and r...

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Autores principales: Haegeman, Andy, De Leeuw, Ilse, Mostin, Laurent, Van Campe, Willem, Aerts, Laetitia, Vastag, Maria, De Clercq, Kris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996284/
https://www.ncbi.nlm.nih.gov/pubmed/31837373
http://dx.doi.org/10.1016/j.jviromet.2019.113800
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author Haegeman, Andy
De Leeuw, Ilse
Mostin, Laurent
Van Campe, Willem
Aerts, Laetitia
Vastag, Maria
De Clercq, Kris
author_facet Haegeman, Andy
De Leeuw, Ilse
Mostin, Laurent
Van Campe, Willem
Aerts, Laetitia
Vastag, Maria
De Clercq, Kris
author_sort Haegeman, Andy
collection PubMed
description During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs.
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spelling pubmed-69962842020-03-01 An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies Haegeman, Andy De Leeuw, Ilse Mostin, Laurent Van Campe, Willem Aerts, Laetitia Vastag, Maria De Clercq, Kris J Virol Methods Article During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs. Elsevier/North-Holland Biomedical Press 2020-03 /pmc/articles/PMC6996284/ /pubmed/31837373 http://dx.doi.org/10.1016/j.jviromet.2019.113800 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haegeman, Andy
De Leeuw, Ilse
Mostin, Laurent
Van Campe, Willem
Aerts, Laetitia
Vastag, Maria
De Clercq, Kris
An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title_full An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title_fullStr An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title_full_unstemmed An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title_short An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies
title_sort immunoperoxidase monolayer assay (ipma) for the detection of lumpy skin disease antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996284/
https://www.ncbi.nlm.nih.gov/pubmed/31837373
http://dx.doi.org/10.1016/j.jviromet.2019.113800
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