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Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer deaths worldwide. The high mortality rate in HCC is largely due to the difficulty of early detection. In this study, to improve patient outcomes, serum samples from 345 patients with HCC, 46 patients with chronic hepatitis (CH), 93...

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Autores principales: Yamamoto, Yusuke, Kondo, Shunsuke, Matsuzaki, Juntaro, Esaki, Minoru, Okusaka, Takuji, Shimada, Kazuaki, Murakami, Yoshiki, Enomoto, Masaru, Tamori, Akihiro, Kato, Ken, Aoki, Yoshiaki, Takizawa, Satoko, Sakamoto, Hiromi, Niida, Shumpei, Takeshita, Fumitaka, Ochiya, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996324/
https://www.ncbi.nlm.nih.gov/pubmed/32025611
http://dx.doi.org/10.1002/hep4.1451
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author Yamamoto, Yusuke
Kondo, Shunsuke
Matsuzaki, Juntaro
Esaki, Minoru
Okusaka, Takuji
Shimada, Kazuaki
Murakami, Yoshiki
Enomoto, Masaru
Tamori, Akihiro
Kato, Ken
Aoki, Yoshiaki
Takizawa, Satoko
Sakamoto, Hiromi
Niida, Shumpei
Takeshita, Fumitaka
Ochiya, Takahiro
author_facet Yamamoto, Yusuke
Kondo, Shunsuke
Matsuzaki, Juntaro
Esaki, Minoru
Okusaka, Takuji
Shimada, Kazuaki
Murakami, Yoshiki
Enomoto, Masaru
Tamori, Akihiro
Kato, Ken
Aoki, Yoshiaki
Takizawa, Satoko
Sakamoto, Hiromi
Niida, Shumpei
Takeshita, Fumitaka
Ochiya, Takahiro
author_sort Yamamoto, Yusuke
collection PubMed
description Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer deaths worldwide. The high mortality rate in HCC is largely due to the difficulty of early detection. In this study, to improve patient outcomes, serum samples from 345 patients with HCC, 46 patients with chronic hepatitis (CH), 93 patients with liver cirrhosis (LC), and 1,033 healthy individuals were analyzed with microRNA (miRNA) microarrays. We investigated the diagnostic potential of circulating miRNAs in serum and developed a detection model of HCC, including early stage. A diagnostic model was constructed based on the expression levels of a combination of miRNAs in a discovery set. We selected 52 miRNAs that had altered expressions according to disease progression status, established the diagnostic model with a combination of eight miRNAs in the discovery set, and tested the model in a validation set. The diagnostic values for discriminating cancer from HCC at‐risk control samples were as follows: area under the curve, 0.99; sensitivity, 97.7%; specificity, 94.7%. With this model, 98% of stage I HCC cases were detected; these results were much better than those observed from conventional methods. Conclusion: Circulating miRNAs could serve as biomarkers for the accurate detection of HCC. Because the diagnostic accuracy was maintained even in stage I, this may represent an accurate detection method even for early stage HCC.
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spelling pubmed-69963242020-02-05 Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease Yamamoto, Yusuke Kondo, Shunsuke Matsuzaki, Juntaro Esaki, Minoru Okusaka, Takuji Shimada, Kazuaki Murakami, Yoshiki Enomoto, Masaru Tamori, Akihiro Kato, Ken Aoki, Yoshiaki Takizawa, Satoko Sakamoto, Hiromi Niida, Shumpei Takeshita, Fumitaka Ochiya, Takahiro Hepatol Commun Original Articles Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer deaths worldwide. The high mortality rate in HCC is largely due to the difficulty of early detection. In this study, to improve patient outcomes, serum samples from 345 patients with HCC, 46 patients with chronic hepatitis (CH), 93 patients with liver cirrhosis (LC), and 1,033 healthy individuals were analyzed with microRNA (miRNA) microarrays. We investigated the diagnostic potential of circulating miRNAs in serum and developed a detection model of HCC, including early stage. A diagnostic model was constructed based on the expression levels of a combination of miRNAs in a discovery set. We selected 52 miRNAs that had altered expressions according to disease progression status, established the diagnostic model with a combination of eight miRNAs in the discovery set, and tested the model in a validation set. The diagnostic values for discriminating cancer from HCC at‐risk control samples were as follows: area under the curve, 0.99; sensitivity, 97.7%; specificity, 94.7%. With this model, 98% of stage I HCC cases were detected; these results were much better than those observed from conventional methods. Conclusion: Circulating miRNAs could serve as biomarkers for the accurate detection of HCC. Because the diagnostic accuracy was maintained even in stage I, this may represent an accurate detection method even for early stage HCC. John Wiley and Sons Inc. 2019-12-19 /pmc/articles/PMC6996324/ /pubmed/32025611 http://dx.doi.org/10.1002/hep4.1451 Text en © 2019 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yamamoto, Yusuke
Kondo, Shunsuke
Matsuzaki, Juntaro
Esaki, Minoru
Okusaka, Takuji
Shimada, Kazuaki
Murakami, Yoshiki
Enomoto, Masaru
Tamori, Akihiro
Kato, Ken
Aoki, Yoshiaki
Takizawa, Satoko
Sakamoto, Hiromi
Niida, Shumpei
Takeshita, Fumitaka
Ochiya, Takahiro
Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title_full Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title_fullStr Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title_full_unstemmed Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title_short Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease
title_sort highly sensitive circulating microrna panel for accurate detection of hepatocellular carcinoma in patients with liver disease
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996324/
https://www.ncbi.nlm.nih.gov/pubmed/32025611
http://dx.doi.org/10.1002/hep4.1451
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