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The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis

Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin‐dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1...

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Autores principales: Yang, Chao, Sofroni, Kostika, Wijnker, Erik, Hamamura, Yuki, Carstens, Lena, Harashima, Hirofumi, Stolze, Sara Christina, Vezon, Daniel, Chelysheva, Liudmila, Orban‐Nemeth, Zsuzsanna, Pochon, Gaëtan, Nakagami, Hirofumi, Schlögelhofer, Peter, Grelon, Mathilde, Schnittger, Arp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996576/
https://www.ncbi.nlm.nih.gov/pubmed/31556459
http://dx.doi.org/10.15252/embj.2019101625
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author Yang, Chao
Sofroni, Kostika
Wijnker, Erik
Hamamura, Yuki
Carstens, Lena
Harashima, Hirofumi
Stolze, Sara Christina
Vezon, Daniel
Chelysheva, Liudmila
Orban‐Nemeth, Zsuzsanna
Pochon, Gaëtan
Nakagami, Hirofumi
Schlögelhofer, Peter
Grelon, Mathilde
Schnittger, Arp
author_facet Yang, Chao
Sofroni, Kostika
Wijnker, Erik
Hamamura, Yuki
Carstens, Lena
Harashima, Hirofumi
Stolze, Sara Christina
Vezon, Daniel
Chelysheva, Liudmila
Orban‐Nemeth, Zsuzsanna
Pochon, Gaëtan
Nakagami, Hirofumi
Schlögelhofer, Peter
Grelon, Mathilde
Schnittger, Arp
author_sort Yang, Chao
collection PubMed
description Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin‐dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA (+) ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self‐polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two‐pronged mechanism to initiate chromosome axis formation in meiosis.
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spelling pubmed-69965762020-02-05 The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis Yang, Chao Sofroni, Kostika Wijnker, Erik Hamamura, Yuki Carstens, Lena Harashima, Hirofumi Stolze, Sara Christina Vezon, Daniel Chelysheva, Liudmila Orban‐Nemeth, Zsuzsanna Pochon, Gaëtan Nakagami, Hirofumi Schlögelhofer, Peter Grelon, Mathilde Schnittger, Arp EMBO J Articles Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin‐dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA (+) ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self‐polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two‐pronged mechanism to initiate chromosome axis formation in meiosis. John Wiley and Sons Inc. 2019-09-26 2020-02-03 /pmc/articles/PMC6996576/ /pubmed/31556459 http://dx.doi.org/10.15252/embj.2019101625 Text en © 2019 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Yang, Chao
Sofroni, Kostika
Wijnker, Erik
Hamamura, Yuki
Carstens, Lena
Harashima, Hirofumi
Stolze, Sara Christina
Vezon, Daniel
Chelysheva, Liudmila
Orban‐Nemeth, Zsuzsanna
Pochon, Gaëtan
Nakagami, Hirofumi
Schlögelhofer, Peter
Grelon, Mathilde
Schnittger, Arp
The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title_full The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title_fullStr The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title_full_unstemmed The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title_short The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
title_sort arabidopsis cdk1/cdk2 homolog cdka;1 controls chromosome axis assembly during plant meiosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996576/
https://www.ncbi.nlm.nih.gov/pubmed/31556459
http://dx.doi.org/10.15252/embj.2019101625
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