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Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum

The amoeba Dictyostelium discoideum has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. Francisella tularensis is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different...

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Autores principales: Kelava, Ina, Marecic, Valentina, Fucak, Petra, Ivek, Elena, Kolaric, Dominik, Ozanic, Mateja, Mihelcic, Mirna, Santic, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996686/
https://www.ncbi.nlm.nih.gov/pubmed/32090107
http://dx.doi.org/10.1155/2020/6826983
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author Kelava, Ina
Marecic, Valentina
Fucak, Petra
Ivek, Elena
Kolaric, Dominik
Ozanic, Mateja
Mihelcic, Mirna
Santic, Marina
author_facet Kelava, Ina
Marecic, Valentina
Fucak, Petra
Ivek, Elena
Kolaric, Dominik
Ozanic, Mateja
Mihelcic, Mirna
Santic, Marina
author_sort Kelava, Ina
collection PubMed
description The amoeba Dictyostelium discoideum has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. Francisella tularensis is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different phagocytic and nonphagocytic cells including mammalian, amoebae, and arthropod cells. The aim of this study was to determine the optimal temperature and infection dose in the interaction of Francisella novicida with D. discoideum in order to establish a model of Francisella infection in the social amoeba. The amoeba cells were infected with a different multiplicity of infection (5, 10, and 100) and incubated at different temperatures (22, 25, 27, 30, and 37°C). The number of intracellular bacteria within D. discoideum, as well as cytotoxicity, was determined at 2, 4, 24, 48, and 72 hours after infection. Our results showed that the optimal temperature for Francisella intracellular replication within amoeba is 30°C with the MOI of 10. We can conclude that this MOI and temperature induced the optimal growth of bacteria in Dictyostelium with low cytotoxicity.
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spelling pubmed-69966862020-02-23 Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum Kelava, Ina Marecic, Valentina Fucak, Petra Ivek, Elena Kolaric, Dominik Ozanic, Mateja Mihelcic, Mirna Santic, Marina Biomed Res Int Research Article The amoeba Dictyostelium discoideum has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. Francisella tularensis is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different phagocytic and nonphagocytic cells including mammalian, amoebae, and arthropod cells. The aim of this study was to determine the optimal temperature and infection dose in the interaction of Francisella novicida with D. discoideum in order to establish a model of Francisella infection in the social amoeba. The amoeba cells were infected with a different multiplicity of infection (5, 10, and 100) and incubated at different temperatures (22, 25, 27, 30, and 37°C). The number of intracellular bacteria within D. discoideum, as well as cytotoxicity, was determined at 2, 4, 24, 48, and 72 hours after infection. Our results showed that the optimal temperature for Francisella intracellular replication within amoeba is 30°C with the MOI of 10. We can conclude that this MOI and temperature induced the optimal growth of bacteria in Dictyostelium with low cytotoxicity. Hindawi 2020-01-20 /pmc/articles/PMC6996686/ /pubmed/32090107 http://dx.doi.org/10.1155/2020/6826983 Text en Copyright © 2020 Ina Kelava et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kelava, Ina
Marecic, Valentina
Fucak, Petra
Ivek, Elena
Kolaric, Dominik
Ozanic, Mateja
Mihelcic, Mirna
Santic, Marina
Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title_full Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title_fullStr Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title_full_unstemmed Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title_short Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
title_sort optimisation of external factors for the growth of francisella novicida within dictyostelium discoideum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996686/
https://www.ncbi.nlm.nih.gov/pubmed/32090107
http://dx.doi.org/10.1155/2020/6826983
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