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Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors

Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myog...

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Autores principales: Choi, In Young, Lim, Hotae, Cho, Hyeon Jin, Oh, Yohan, Chou, Bin-Kuan, Bai, Hao, Cheng, Linzhao, Kim, Yong Jun, Hyun, SangHwan, Kim, Hyesoo, Shin, Joo Heon, Lee, Gabsang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996923/
https://www.ncbi.nlm.nih.gov/pubmed/32011235
http://dx.doi.org/10.7554/eLife.46981
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author Choi, In Young
Lim, Hotae
Cho, Hyeon Jin
Oh, Yohan
Chou, Bin-Kuan
Bai, Hao
Cheng, Linzhao
Kim, Yong Jun
Hyun, SangHwan
Kim, Hyesoo
Shin, Joo Heon
Lee, Gabsang
author_facet Choi, In Young
Lim, Hotae
Cho, Hyeon Jin
Oh, Yohan
Chou, Bin-Kuan
Bai, Hao
Cheng, Linzhao
Kim, Yong Jun
Hyun, SangHwan
Kim, Hyesoo
Shin, Joo Heon
Lee, Gabsang
author_sort Choi, In Young
collection PubMed
description Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny (online database can be accessed in http://www.myogenesis.net/).
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spelling pubmed-69969232020-02-05 Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors Choi, In Young Lim, Hotae Cho, Hyeon Jin Oh, Yohan Chou, Bin-Kuan Bai, Hao Cheng, Linzhao Kim, Yong Jun Hyun, SangHwan Kim, Hyesoo Shin, Joo Heon Lee, Gabsang eLife Stem Cells and Regenerative Medicine Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny (online database can be accessed in http://www.myogenesis.net/). eLife Sciences Publications, Ltd 2020-02-03 /pmc/articles/PMC6996923/ /pubmed/32011235 http://dx.doi.org/10.7554/eLife.46981 Text en © 2020, Choi et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Stem Cells and Regenerative Medicine
Choi, In Young
Lim, Hotae
Cho, Hyeon Jin
Oh, Yohan
Chou, Bin-Kuan
Bai, Hao
Cheng, Linzhao
Kim, Yong Jun
Hyun, SangHwan
Kim, Hyesoo
Shin, Joo Heon
Lee, Gabsang
Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title_full Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title_fullStr Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title_full_unstemmed Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title_short Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
title_sort transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of twist1 in maintenance of skeletal muscle progenitors
topic Stem Cells and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996923/
https://www.ncbi.nlm.nih.gov/pubmed/32011235
http://dx.doi.org/10.7554/eLife.46981
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