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Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer

OBJECTIVE: To investigate the expression and evaluate the clinical significance of long non-coding RNA, LINC01124, in non-small cell lung cancer (NSCLC) and to study its influence in this tumor. METHODS: Hundred specimens of NSCLC tissues and normal lung tissues after surgery were collected. The qRT...

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Autores principales: Wang, Zi-Bo, Zhang, Hong-Yan, Lu, Ji-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997214/
https://www.ncbi.nlm.nih.gov/pubmed/32099381
http://dx.doi.org/10.2147/OTT.S214049
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author Wang, Zi-Bo
Zhang, Hong-Yan
Lu, Ji-Bin
author_facet Wang, Zi-Bo
Zhang, Hong-Yan
Lu, Ji-Bin
author_sort Wang, Zi-Bo
collection PubMed
description OBJECTIVE: To investigate the expression and evaluate the clinical significance of long non-coding RNA, LINC01124, in non-small cell lung cancer (NSCLC) and to study its influence in this tumor. METHODS: Hundred specimens of NSCLC tissues and normal lung tissues after surgery were collected. The qRT-PCR for LINC01124 expression was performed on cancerous and normal lung tissues. The correlations between the expression of LINC01124 and pathological characteristics were analyzed. PcDNA-LINC01124 was transfected to upregulate LINC01124 expression in NSCLC cells, and the transfection efficiency was evaluated by the qRT-PCR. CCK8 assay, wound-healing assay, and the Transwell assay were performed to evaluate the effect of ectopic LINC01124 expression on proliferation, migration, and invasive of NSCLC cells. RESULTS: The expression level of LINC01124 was downregulated in tumor tissues when compared with the paired normal lung tissues (P<0.05). The expression of LINC01124 was associated with patients’ age and distant metastasis (P<0.05). Enforced expression of LINC01124 significantly inhibited the proliferation, migration, and invasive ability of NSCLC cells. CONCLUSION: The expression of LINC01124 was decreased in patients with NSCLC of older age and with those having distant metastasis. LINC01124 may inhibit cell proliferation, migration, and invasive ability.
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spelling pubmed-69972142020-02-25 Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer Wang, Zi-Bo Zhang, Hong-Yan Lu, Ji-Bin Onco Targets Ther Original Research OBJECTIVE: To investigate the expression and evaluate the clinical significance of long non-coding RNA, LINC01124, in non-small cell lung cancer (NSCLC) and to study its influence in this tumor. METHODS: Hundred specimens of NSCLC tissues and normal lung tissues after surgery were collected. The qRT-PCR for LINC01124 expression was performed on cancerous and normal lung tissues. The correlations between the expression of LINC01124 and pathological characteristics were analyzed. PcDNA-LINC01124 was transfected to upregulate LINC01124 expression in NSCLC cells, and the transfection efficiency was evaluated by the qRT-PCR. CCK8 assay, wound-healing assay, and the Transwell assay were performed to evaluate the effect of ectopic LINC01124 expression on proliferation, migration, and invasive of NSCLC cells. RESULTS: The expression level of LINC01124 was downregulated in tumor tissues when compared with the paired normal lung tissues (P<0.05). The expression of LINC01124 was associated with patients’ age and distant metastasis (P<0.05). Enforced expression of LINC01124 significantly inhibited the proliferation, migration, and invasive ability of NSCLC cells. CONCLUSION: The expression of LINC01124 was decreased in patients with NSCLC of older age and with those having distant metastasis. LINC01124 may inhibit cell proliferation, migration, and invasive ability. Dove 2019-12-31 /pmc/articles/PMC6997214/ /pubmed/32099381 http://dx.doi.org/10.2147/OTT.S214049 Text en © 2019 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Zi-Bo
Zhang, Hong-Yan
Lu, Ji-Bin
Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title_full Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title_fullStr Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title_full_unstemmed Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title_short Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
title_sort expression and effects of long non-coding rna, linc01124, in non-small cell lung cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997214/
https://www.ncbi.nlm.nih.gov/pubmed/32099381
http://dx.doi.org/10.2147/OTT.S214049
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