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Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells

Erythropoietin receptors (EPORs) are present not only in erythrocyte precursors but also in non-hematopoietic cells including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in culture medium on the EPOR expression and viability of the estrogen receptor (ER)-positiv...

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Autores principales: Teo, Guan-Young, Rasedee, Abdullah, AL-Haj, Nagi. A., Beh, Chaw Yee, How, Chee Wun, Rahman, Heshu Sulaiman, Alitheen, Noorjahan Banu, Rosli, Rozita, Abdullah, Al-Shwyeh Hussah, Ali, Abdelwahid Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997850/
https://www.ncbi.nlm.nih.gov/pubmed/32210684
http://dx.doi.org/10.1016/j.sjbs.2019.11.032
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author Teo, Guan-Young
Rasedee, Abdullah
AL-Haj, Nagi. A.
Beh, Chaw Yee
How, Chee Wun
Rahman, Heshu Sulaiman
Alitheen, Noorjahan Banu
Rosli, Rozita
Abdullah, Al-Shwyeh Hussah
Ali, Abdelwahid Saeed
author_facet Teo, Guan-Young
Rasedee, Abdullah
AL-Haj, Nagi. A.
Beh, Chaw Yee
How, Chee Wun
Rahman, Heshu Sulaiman
Alitheen, Noorjahan Banu
Rosli, Rozita
Abdullah, Al-Shwyeh Hussah
Ali, Abdelwahid Saeed
author_sort Teo, Guan-Young
collection PubMed
description Erythropoietin receptors (EPORs) are present not only in erythrocyte precursors but also in non-hematopoietic cells including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in culture medium on the EPOR expression and viability of the estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells. Using flow cytometry, we showed that the inclusion of 10% FBS in the medium increased the EPOR expressions and viabilities of MDA-MB-231 and MCF-7 cells. The MDA-MB-231 showed greater EPOR expression than MCF-7 cells, suggesting that the presence of ERs on cells is associated with poor expression of EPOR. Culture medium containing 10% FBS also caused increased number of breast cancer cells entering the synthesis phase of the cell cycle. The study also showed that rHuEPO treatment did not affect viability of breast cancer cells. In conclusion, it was shown that the inclusion of FBS in culture medium increased expression of EPOR in breast cancer cells and rHuEPO treatment had no effect on the proliferation of these cancer cells.
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spelling pubmed-69978502020-03-24 Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells Teo, Guan-Young Rasedee, Abdullah AL-Haj, Nagi. A. Beh, Chaw Yee How, Chee Wun Rahman, Heshu Sulaiman Alitheen, Noorjahan Banu Rosli, Rozita Abdullah, Al-Shwyeh Hussah Ali, Abdelwahid Saeed Saudi J Biol Sci Article Erythropoietin receptors (EPORs) are present not only in erythrocyte precursors but also in non-hematopoietic cells including cancer cells. In this study, we determined the effect of fetal bovine serum (FBS) in culture medium on the EPOR expression and viability of the estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells. Using flow cytometry, we showed that the inclusion of 10% FBS in the medium increased the EPOR expressions and viabilities of MDA-MB-231 and MCF-7 cells. The MDA-MB-231 showed greater EPOR expression than MCF-7 cells, suggesting that the presence of ERs on cells is associated with poor expression of EPOR. Culture medium containing 10% FBS also caused increased number of breast cancer cells entering the synthesis phase of the cell cycle. The study also showed that rHuEPO treatment did not affect viability of breast cancer cells. In conclusion, it was shown that the inclusion of FBS in culture medium increased expression of EPOR in breast cancer cells and rHuEPO treatment had no effect on the proliferation of these cancer cells. Elsevier 2020-02 2019-12-06 /pmc/articles/PMC6997850/ /pubmed/32210684 http://dx.doi.org/10.1016/j.sjbs.2019.11.032 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Teo, Guan-Young
Rasedee, Abdullah
AL-Haj, Nagi. A.
Beh, Chaw Yee
How, Chee Wun
Rahman, Heshu Sulaiman
Alitheen, Noorjahan Banu
Rosli, Rozita
Abdullah, Al-Shwyeh Hussah
Ali, Abdelwahid Saeed
Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title_full Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title_fullStr Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title_full_unstemmed Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title_short Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
title_sort effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997850/
https://www.ncbi.nlm.nih.gov/pubmed/32210684
http://dx.doi.org/10.1016/j.sjbs.2019.11.032
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