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Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells

The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal models of M...

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Autores principales: Rodríguez-Lorenzo, Sabela, Konings, Julia, van der Pol, Susanne, Kamermans, Alwin, Amor, Sandra, van Horssen, Jack, Witte, Maarten E., Kooij, Gijs, de Vries, Helga E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998074/
https://www.ncbi.nlm.nih.gov/pubmed/32014066
http://dx.doi.org/10.1186/s40478-020-0885-1
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author Rodríguez-Lorenzo, Sabela
Konings, Julia
van der Pol, Susanne
Kamermans, Alwin
Amor, Sandra
van Horssen, Jack
Witte, Maarten E.
Kooij, Gijs
de Vries, Helga E.
author_facet Rodríguez-Lorenzo, Sabela
Konings, Julia
van der Pol, Susanne
Kamermans, Alwin
Amor, Sandra
van Horssen, Jack
Witte, Maarten E.
Kooij, Gijs
de Vries, Helga E.
author_sort Rodríguez-Lorenzo, Sabela
collection PubMed
description The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal models of MS pointed to the CP as a key route for immune cell invasion of the CNS, little is known about the distribution of immune cells in the human CP during progressive phases of MS. Here, we use immunohistochemistry and confocal microscopy to explore the main immune cell populations in the CP of progressive MS patients and non-neuroinflammatory controls, in terms of abundance and location within the distinct CP compartments. We show for the first time that the CP stromal density of granulocytes and CD8+ T cells is higher in progressive MS patients compared to controls. In line with previous studies, the CP of both controls and progressive MS patients contains relatively high numbers of macrophages and dendritic cells. Moreover, we found virtually no B cells or plasma cells in the CP. MHCII+ antigen-presenting cells were often found in close proximity to T cells, suggesting constitutive CNS immune monitoring functions of the CP. Together, our data highlights the role of the CP in immune homeostasis and indicates the occurrence of mild inflammatory processes in the CP of progressive MS patients. However, our findings suggest that the CP is only marginally involved in immune cell migration into the CNS in chronic MS.
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spelling pubmed-69980742020-02-05 Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells Rodríguez-Lorenzo, Sabela Konings, Julia van der Pol, Susanne Kamermans, Alwin Amor, Sandra van Horssen, Jack Witte, Maarten E. Kooij, Gijs de Vries, Helga E. Acta Neuropathol Commun Research The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal models of MS pointed to the CP as a key route for immune cell invasion of the CNS, little is known about the distribution of immune cells in the human CP during progressive phases of MS. Here, we use immunohistochemistry and confocal microscopy to explore the main immune cell populations in the CP of progressive MS patients and non-neuroinflammatory controls, in terms of abundance and location within the distinct CP compartments. We show for the first time that the CP stromal density of granulocytes and CD8+ T cells is higher in progressive MS patients compared to controls. In line with previous studies, the CP of both controls and progressive MS patients contains relatively high numbers of macrophages and dendritic cells. Moreover, we found virtually no B cells or plasma cells in the CP. MHCII+ antigen-presenting cells were often found in close proximity to T cells, suggesting constitutive CNS immune monitoring functions of the CP. Together, our data highlights the role of the CP in immune homeostasis and indicates the occurrence of mild inflammatory processes in the CP of progressive MS patients. However, our findings suggest that the CP is only marginally involved in immune cell migration into the CNS in chronic MS. BioMed Central 2020-02-03 /pmc/articles/PMC6998074/ /pubmed/32014066 http://dx.doi.org/10.1186/s40478-020-0885-1 Text en © The Author(s). 2020, corrected publication 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rodríguez-Lorenzo, Sabela
Konings, Julia
van der Pol, Susanne
Kamermans, Alwin
Amor, Sandra
van Horssen, Jack
Witte, Maarten E.
Kooij, Gijs
de Vries, Helga E.
Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title_full Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title_fullStr Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title_full_unstemmed Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title_short Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
title_sort inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and t cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998074/
https://www.ncbi.nlm.nih.gov/pubmed/32014066
http://dx.doi.org/10.1186/s40478-020-0885-1
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