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A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity
Innate immunity is an evolutionarily conserved host defense system against infections. The fruit fly Drosophila relies solely on innate immunity for infection defense, and the conservation of innate immunity makes Drosophila an ideal model for understanding the principles of innate immunity, which c...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999089/ https://www.ncbi.nlm.nih.gov/pubmed/32063902 http://dx.doi.org/10.3389/fimmu.2020.00035 |
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author | Iwashita, Shinzo Suzuki, Hiroaki Goto, Akira Oyama, Tomohito Kanoh, Hirotaka Kuraishi, Takayuki Fuse, Naoyuki Yano, Tamaki Oshima, Yoshiteru Dow, Julian A. T. Davies, Shireen-Anne Kurata, Shoichiro |
author_facet | Iwashita, Shinzo Suzuki, Hiroaki Goto, Akira Oyama, Tomohito Kanoh, Hirotaka Kuraishi, Takayuki Fuse, Naoyuki Yano, Tamaki Oshima, Yoshiteru Dow, Julian A. T. Davies, Shireen-Anne Kurata, Shoichiro |
author_sort | Iwashita, Shinzo |
collection | PubMed |
description | Innate immunity is an evolutionarily conserved host defense system against infections. The fruit fly Drosophila relies solely on innate immunity for infection defense, and the conservation of innate immunity makes Drosophila an ideal model for understanding the principles of innate immunity, which comprises both humoral and cellular responses. The mechanisms underlying the coordination of humoral and cellular responses, however, has remained unclear. Previously, we identified Gyc76C, a receptor-type guanylate cyclase that produces cyclic guanosine monophosphate (cGMP), as an immune receptor in Drosophila. Gyc76C mediates the induction of antimicrobial peptides for humoral responses by a novel cGMP pathway including a membrane-localized cGMP-dependent protein kinase, DG2, through downstream components of the Toll receptor such as dMyD88. Here we show that Gyc76C is also required for the proliferation of blood cells (hemocytes) for cellular responses to bacterial infections. In contrast to Gyc76C-dependent antimicrobial peptide induction, Gyc76C-dependent hemocyte proliferation is meditated by a small GTPase, Ras85D, and not by DG2 or dMyD88, indicating that Gyc76C mediates the cellular and humoral immune responses in distinct ways. |
format | Online Article Text |
id | pubmed-6999089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69990892020-02-14 A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity Iwashita, Shinzo Suzuki, Hiroaki Goto, Akira Oyama, Tomohito Kanoh, Hirotaka Kuraishi, Takayuki Fuse, Naoyuki Yano, Tamaki Oshima, Yoshiteru Dow, Julian A. T. Davies, Shireen-Anne Kurata, Shoichiro Front Immunol Immunology Innate immunity is an evolutionarily conserved host defense system against infections. The fruit fly Drosophila relies solely on innate immunity for infection defense, and the conservation of innate immunity makes Drosophila an ideal model for understanding the principles of innate immunity, which comprises both humoral and cellular responses. The mechanisms underlying the coordination of humoral and cellular responses, however, has remained unclear. Previously, we identified Gyc76C, a receptor-type guanylate cyclase that produces cyclic guanosine monophosphate (cGMP), as an immune receptor in Drosophila. Gyc76C mediates the induction of antimicrobial peptides for humoral responses by a novel cGMP pathway including a membrane-localized cGMP-dependent protein kinase, DG2, through downstream components of the Toll receptor such as dMyD88. Here we show that Gyc76C is also required for the proliferation of blood cells (hemocytes) for cellular responses to bacterial infections. In contrast to Gyc76C-dependent antimicrobial peptide induction, Gyc76C-dependent hemocyte proliferation is meditated by a small GTPase, Ras85D, and not by DG2 or dMyD88, indicating that Gyc76C mediates the cellular and humoral immune responses in distinct ways. Frontiers Media S.A. 2020-01-28 /pmc/articles/PMC6999089/ /pubmed/32063902 http://dx.doi.org/10.3389/fimmu.2020.00035 Text en Copyright © 2020 Iwashita, Suzuki, Goto, Oyama, Kanoh, Kuraishi, Fuse, Yano, Oshima, Dow, Davies and Kurata. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Iwashita, Shinzo Suzuki, Hiroaki Goto, Akira Oyama, Tomohito Kanoh, Hirotaka Kuraishi, Takayuki Fuse, Naoyuki Yano, Tamaki Oshima, Yoshiteru Dow, Julian A. T. Davies, Shireen-Anne Kurata, Shoichiro A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title | A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title_full | A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title_fullStr | A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title_full_unstemmed | A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title_short | A Receptor Guanylate Cyclase, Gyc76C, Mediates Humoral, and Cellular Responses in Distinct Ways in Drosophila Immunity |
title_sort | receptor guanylate cyclase, gyc76c, mediates humoral, and cellular responses in distinct ways in drosophila immunity |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999089/ https://www.ncbi.nlm.nih.gov/pubmed/32063902 http://dx.doi.org/10.3389/fimmu.2020.00035 |
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